Carbohydrate Metabolism Flashcards
Describe the energy cycle in life.
Describe the relationships between gluconeogenesis, pentose phosphate pathway, glycogen metabolism and oxidative phosphorylation.
Describe the role of oxygen in harvesting food energy.
Most eukaryote cells make ATP in the mitochondria.
Not all cells have mitochondria. e.g., red blood cells.
Describe the metabolism of glucose.
How does glycolysis alone make some ATP?
What is the glycolysis reaction?
- the uptake of glucose by the enzyme hexokinase.
- hexokinase has high affinity for glucose (low km)
- glucose is phosphorylated to G-6-Phosphate.
- Glucose is trapped in cell as now negatively charged (ATP is hosphate donor).
- ATP is used during investment phase (energetically unfavourable reactions).
- Electrons are released during payoff.
- Electronsa re transferred to NAD and makes NADH
- Usually referred to as substrate level phosphorylation (not oxidative as glycolysis does not require oxygen).
how does hormones and enzymes regulate glycolysis?
- the regulation of glycolysis by activation / inhibition of rate-limiting enzymes is short term (minutes-hours).
- Glycolysis also regulated by hormones-insulin.
- Hormonal control is slower and often more pronounced by influencing the amount of enzyme synthesised (10-20 x increase in enzymes over hours-days).
What are some alternative fates of pyruvate?
- if glycolysis continues, electrons released will eventually “saturate” all the available NAD converting it to NADH. Glycolysis would stop = fatal!
- Regeneration of NAD occurs by making ethanol (fermentation) or lactic acid.
- oxidative phosphorylation -> much greater levels of ATP produced and requires oxygen.
Krebs Cycle Overview
- AKA - tricarboxylic or citric acid cycle.
- oxidation of acetyl-CoA to CO2 & H2O.
- occurs in mitochondrial matrix - electron transport chain (ETC).
- number of roles in metabolism.
- important in anabolism - glucose, ketones, AAs & heme synthesis.
Krebs Cycle - regualtion
- availability of ADP -> ATP production matches that consumption - e.g., resting Vs exercise
- Activation and inhibition of allosteric enzyme activities.
Pentose Phosphate Pathway - Overview
- Occurs in cytosol
- no ATP consumed or produced.
- G-6P <-> Ribose 5-P + CO2 + 2NADPH
- Rate and direction of reactions determined by intermediates NADPH (biochemical reductant):
- steroid synthesis (Adrenal cortex)
- reduction of reactive oxygen intermediates
- Detoxification (liver cytochrome P-450)
- respiratory burst in phagocytic cells - Ribose 5-P -synthesis of nucleotides
Pentose Phosphate Pathway - Reactions
Glycogen metabolism - overview
- Human require a constant supply of BG
- preferred energy source for brain
- energy source for cells with few or no mitochondria e.g., RBCs
- energy source for exercising muscle (anaerobic glycolysis) - Blood glucose obtained from:
- diet: sporadic and not reliable
- gluconeogenesis: can supply sustained synthesis, but slow to respond to decreased blood glucose level (BGL).
- glycogen: glucose from liver glycogen (maintains BGL)
- muscle glycogen releases glucose during exercise for the muscle.
liver glycogen increase during well-fed state, and decreases during fast.
muscle glycogen only moderate depleted during prolonged fasting.
Glycogen metabolism - synthesis
glycogen metabolism - breakdown