Ca2+ Imaging

Question 1

Green Fluorescent protein

Answer 1

proteins that are structural homologs of Aequorea green fluorescent protein and that are able to form an internal visible wavelength fluorophore from their own polypeptide sequence

When exposed to blue or uv light it emits a green fluorescent light

Question 2

GcaMP

Answer 2

It is a protein that serves as a calcium

composed of:
circularly permuted enhanced GFP moiety attached to ->
the calcium binding protein calmodulin and -> the CaM binding peptide M13pep

Question 3

How GFP helps understand neuronal structure

Answer 3

Biologists introduce a gene encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy

Question 4

How is Ca2+ imaging being used for imagining of neuronal activity in freely moving animals

Answer 4

small enough to implant in the head of a living mouse and gather images from its brain without hindering its movement. has potential for tracking population engaged in specific behavior

Question 5

Fluorescence

Answer 5

A from of luminescence. The emission of a long-wavelength that (lower-energy) by a substance that has absorbed shorter-wavelength (higher-energy, like Xrays) light