C7: Biotechnology

Question 1

Question: Why is the DNA of the bacterium not cut by restriction endonucleases?

Answer 1

Answer: The DNA of the bacterium is not cut by restriction endonucleases because methylase enzymes modify the recognition sites.

Question 2

Question: What are sticky ends?

Answer 2

Answer: Sticky ends are short single-stranded lengths of DNA that stick out from double-stranded DNA after it has been cut using certain restriction endonucleases.