Burkholderia (Lec4)

Question 1

Are there any similarities between structure and sequences of BPSL1594 and CNF1?

Answer 1

Whilst v little sequence homology, both enzymes take up a reasonably similar shape and both contain a His-Cys pair in the AS

Question 2

How are CNF1 and BPSL1594 classified as toxins? ie what are their mode of actions

Draw the structure of this deamidation

Answer 2

Both deamidate Gln -> Glu in CNF1 this occurs in a GTPase eg Rho so that it is constitutively active in BPSL1594 occurs in an RNA helicase (eIF4A), loses its activity, translation cannot occur

Question 3

What was the name of BPSL1594 changed to?

Answer 3

Burkholderia lethal factor 1 (BLF1)

Question 4

Fill in the rest of this diagram

Answer 4

Question 5

Why does CNF1 from E. coli have these extra domains when compared to BLF1?

Answer 5

these additional domains allow E. coli to secrete CNF1 and for it to successfully cross the eukaryotic cell membrane to enter the cytoplasm

due to the intracelleular nature of Burkholderia it is excreted straight into the cytoplasm