Lec 7 - Medium Chain Alcohol Dehydrogenase Flashcards
1
Q
Give the reaction that is catalysed by glucose dehydrogenase and state which cofactor is used
A
glucose + NAD(P)+ –> gluconolactone + NAD(P)H + H+
Zn ion cofactor
2
Q
Name the residues that are used to coordinate the Zn ion
A
Glutamate and His residues
3
Q
Does the GlcDH enzyme remain static during catalysis?
A
No the AS changes conformation continuously as the residues that coordinate the Zn and H20 etc differ
4
Q
Give the number of bonds Zn makes at its maximum with various residues and the product etc
A
Zn is coordinated by a max of 5 residues
5
Q
Give 2 features of a good inhibitor
A
- complementarity to the AS
- mimics the TS of the substrate
