bunyaviridae

Question 1

bunyaviridae baltimore classification

Answer 1

classification V (ss- RNA)

Question 2

types of ss- RNA viruses

Answer 2

Rhabdoviridae, paramyxoviridae, filoviridae (non-segmented). Arenaviridae, bunyaviridae, orthomyxoviridae (segmented genome).

Question 3

Difference between + and - RNA viruses

Answer 3

plus strand: infectious, directly translatable.

minus strand: not infectious, not translatable

Question 4

family of bunyairidae

Answer 4

orthobunyavirus, phlebovirus (rift valley fever), hantavirus (Hantaan), Nairovirus, Tospovirus (TSV)

Question 5

Bunyaviridae virus features

Answer 5

spherical, enveloped virus particles (80-120 nm)
ss- RNA genome
segmented, tripartite RNA genome (S,M,L RNA)
particle assembly at golgi membrane
cytoplasmic replication

Question 6

bunyaviridae virus particle

Answer 6

structural proteins
nucleoprotein
glycoprotein Gn and Gc. Core consists of ribonucleoproteins (RNPs).

Question 7

Genome organization and expression strategy

Answer 7

L M S. L contains RdRp. M contains NSm and Gn-Gc. S contains Nucleoprotein (N) and NSs. Ambisense gene arrangement.

Question 8

Ambisense

Answer 8

where genes are located on the opposite strand, (vRNA and vcRNA). These are not overlapping each other.

Question 9

Transcription initiation of bunyaviridae

Answer 9

Cap-snatching. Capped-leader RNAs are snatched from host mRNAs to initiate transcription on the viral genome. find a host mRNA, cleaves with endonuclease and elongates the strand.

Question 10

function of NSm and NSs

Answer 10

NSm is a movement protein for the plant viruses. NSs is an RNAi protein.