Brucella Flashcards
What disease does Brucellosis affect?
It affects the reproductive organs and reticuloendothelial tissue.
Who discovered the first genus member of Brucella?
David Bruce.
What is the first genus member of Brucella?
Brucella melitensis.
Who discovered Brucella suis, Brucella ovis, and Brucella canis?
Jacob Traum.
Who discovered Brucella abortus?
Frederick Bang.
Describe Brucella abortus.
It is a coccobacillus, facultative intracellular bacterium, non-acid fast, and non-motile.
What is another name for Brucella abortus?
Bangs bacillus.
What are some characteristics of Brucella abortus?
Aerobic, produces H₂S, non-hemophilic, and does not liquefy gelatin.
True or False: Growth of Brucella abortus is not enhanced by blood or serum.
False.
How long does it take for Brucella abortus to reach its maximum size?
5 to 7 days.
True or False: On primary isolation, Brucella is slow-growing and barely visible at 48 hours.
True.
True or False: Growth of Brucella abortus in fluid is sparse.
True.
What does RTD stand for in phage typing?
Minimum number of phages that will produce confluent lysis.
What is bacteriophage typing used for?
It is a rapid and useful method for identifying Brucella abortus.*
What composes the outer layer of Brucella abortus?
Lipopolysaccharide.
What surface antigens are carried on the polysaccharide chains for agglutination reactions?
A and M antigens.
How is Brucella abortus infection acquired?
Ingestion of contaminated food.
What protein stimulates delayed hypersensitivity?
Porin.
What are the transmission routes of Brucella abortus?
Intermammary and congenital transmission.
True or False: Brucella abortus is resistant to sunlight and drying.
False.
True or False: Brucella abortus survives in milk but not pasteurization.
True.
What are some risk factors for Brucella abortus infection?
Community pastures, contamination of streams, and presence of infected animals.
Although male cattle are resistant, how can they transmit Brucella abortus?
Through semen.
What are the portals of entry for Brucella abortus?
Oral mucosa, nasopharynx, conjunctiva, and genital tract.
What is Brucella abortus highly resistant to?
Intraleukocytic killing.
What causes Brucella abortus to proliferate in cells?
Increase in erythritol.
What condition does Brucella abortus cause after penetrating the chorion?
Placentitis.
What is a discernible sign of Brucella abortus infection?
Hygroma of the knee.
Aside from cell-mediated immunity and T lymphocytes, what are other immune responses to Brucella abortus?
Hyperimmune serum and opsonic antibodies.
What strain is used for the live vaccine of Brucella abortus?
Strain 19.
What is the inactivated vaccine for Brucella abortus?
MacEwen.
What culture media is used for diagnosing Brucella abortus?
Tryptose or albumin agar.
What serological test detects IgM in Brucella abortus?
Rose Bengal Plate Test.
What serological test may yield false positives or negatives and is influenced by vaccination?
Serum tube agglutination test.
What test detects Brucella strain 1119-3 and IgM?
Card test.
What test uses gentian violet and brilliant green?
Plate agglutination or rapid test.
What is the principle of the Milk Ring Test?
Hematoxylin-stained suspension added to fresh milk.
In a positive Milk Ring Test, what is observed?
Bluish violet cream layer and decolorized milk.
What is the most accurate serological test for IgM and IgG in Brucella abortus?
Complement fixation test.
What test identifies only IgG antibodies?
Rivanol and mercaptoethanol tests.
