Botany Lab Flashcards
Importance of Plant to Human and animals
- Food
- Fiber
- Fuel
- Medicine
Microscope is invented by:
Gallleo Galloi
Zaccharias Jansen & Hans Lipperhey
Microscope is invented by:
Gallleo Galloi
Zaccharias Jansen & Hans Lipperhey
Uses of micrascope
Botanical Field
Biological Field
Crime Investigation
Educational Field
Medical Field
Types of microscope.
Simple Microscope
2 fight microscope a Compound
b. Desecting (stereomicroscop
3 electron microscope
Transtion Electron Microscope
Scanning Electron Microscope
Scanning Tunneling Microscopi
The chromosome are at the poles, and are becoming more diffuse. The nuclear envelope reforming
The
Telophase
The chromatids of each chromosome have separated and are moving toward the poles.
Anaphase
The chromatids of each chromosome have separated and are moving toward the poles.
Anaphase
Thick colled chromosomes, each with two chromatids, are lined up on the metaphase plate.
Metaphase
an instrument that can magnify images of cells and tissues up to hundreds or even thousand times of their actual size.
Microscope
First perfected the device known as microscope
Galileo Galileo
first men to develop the concept of compound microscope
Zaccharias Janssen & Hans Lipperhey –
– require most material being examined to be sliced thinly enough for the light to pass through.
a. Compound
allow three dimensional viewing of opaque objects.
b. Dissecting (stereomicroscope) –
detailed images of tiny structures within the cell)
- electron microscope
can magnify up to 200, 000 and more.
Transition Electron Microscope -
urface detail of thick objects can be observe when the scanner makes the object visible on a cathode tube like a TV screen.
Scanning Electron Microscope – s
uses minute probe that tunnels electrons upon a sample.
Scanning Tunneling Microscope –
uses minute probe that tunnels electrons upon a sample.
Scanning Tunneling Microscope –
1.Separates the objective and the eyepiece and assures continuous alignment of the optics.
Body tube
2.Holds the objective lenses
3
Revolving nosepiece
- Magnifies 10x and is useful for examining large specimens or surveying many smaller specimens
Low power objective lens
4.Shortest objective and is useful for getting a general overview of a slide.
Scanning objective lens
Magnifies 40x and is ideal for observing very fine detail.
High-power objective lens
6.Holds the slide in place
Stage clip
7.regulates the amount of light on the specimen
Diaphragm
7.regulates the amount of light on the specimen
Diaphragm
8.projects light upward through the diaphragm, the specimen and the lenses.
Light source
9.Contains the ocular lens, used to view specimen
Eyepiece
10.Used to support the microscope when carried
Arm
- Supports the slides being viewed
Stage
12.Moves the stage up and down for focusing
Coarse adjustment knob
13.Moves the stage slightly to sharpen image
Fine adjustment knob
- Supports the microscope
Base
- Supports the microscope
Base
Describe how to properly carry a microscope from one place to another.
Carry with both hands. Grasp the arm with one hand and place the other hand
under the base for support.
How do you properly focus on a specimen?
a. Move the stage down to its lowest position. b. Place the glass slide onto the stage. Be careful pushing it under the clips
that the cover slide doesn’t move or crack.
C. Select the lowest power objective lens. Use the lowest magnification first to
achieve best focus.
d. Turn the coarse focus knob slowly until you can see the cells.
e. Turn the fine focus knob slowly until the cells are in focus and you can see them
clearly.
How do you properly focus on a specimen?
a. Move the stage down to its lowest position. b. Place the glass slide onto the stage. Be careful pushing it under the clips
that the cover slide doesn’t move or crack.
C. Select the lowest power objective lens. Use the lowest magnification first to
achieve best focus.
d. Turn the coarse focus knob slowly until you can see the cells.
e. Turn the fine focus knob slowly until the cells are in focus and you can see them
clearly.
- Some microscopes have a fourth lens called the oil immersion objective (010).
How is this used and how much is the magnifying power?
Oil immersion objective is used for examining details of individual cells (e.g. red
blood cells). Its magnifying power is 1000x.
- Discuss parfocal lenses.
Parfocal lens is a lens that stays in focus when magnification/focal length is
changed.
A person tells you to close one eye when looking through a microscope so that you will properly see the object. Will you follow the person’s advice? Why or
why not?
Do not follow the person’s advice. Look through the microscope with both eyes
open to avoid eye strain.