booklet 1 - cell biology and microscopes Flashcards
how does a light microscope work?
uses light to illuminate objects under view, convex lens manipulates how light enters the eye
how does an electronic microscope work?
lens replaced by electromagnets that ‘bend’ the electron beam. forms an image as a photo - elctron micrograph
radiation source of electron microscope
Electrons
radiation source of light microscope
visible light
max magnification of electron microscope
approx ×1 000 000
max magnification of light microscope
approx ×2 000
resolution of electron microscope
approx 0.1nm
resolution of light microscope
approx 200nm
images seen through electron microscope- colour
black and white
images seen through light microscope- colour and why
coloured - colour is a property of loght
types of electron microscope
TEM and SEM
TEM microscope meaning
transmission electron microscope
how are images seen through a tem and why?
2D - electron beams pass through thin slices of specimen, analyses inner structures
how are images seen through a sem and why?
3D - Specimen coated in gold so electrons bounce off and give outside view
why does standard form exist?
to write down really big or small numbers
how do you write big numbers in standard form?
1 × 10^3 = 1 × 1000= 1000
how do you write small numbers in standard form?
1 × 10^-2 = 0.01