Body system Flashcards
What happens on day 1
receiving the sample
-compare req to sample
-plant the sample
◦ Specimen number
◦ Name of patient
◦ Patient’s hospital ID
◦ Date of birth
◦ Type of specimen
What happens if it doenst match?
on day 1 what do you look at when reading the gram
how is the slide set up
-fluid
draw a circle under the slide so its seen on the other side when you put a drop of sample
swab
-roll from side to side
-must air dry and heat fixe
-MUST report on day 1
When reading
Quantitate pus cells
Quantitate epithelial cells - contamination
Quantitate bacteria
When is india ink done on CSF
when you see yeast in direct gram stain
What is done on day 2
Setting up ID and susceptibility tests
-sort PER specimen and EACH isolate gets a separate number
-preliminary reporting for each isolate number
-direct gram should match the specimen
-if not repeat the gram
-do tests that need the 2 hour incubation so theres enough time to read
-check if the result needs a prelim report and call to DR or PHL
on day 2 how do you know what tests to do
go off the CMI, growth on selective media and gram stain to see what ID tests need to be done
-only do the ID and AST listed in COMMON ISOLATE GUIDE
-if you dont have enough isolated colonies for tests that need a suspension AND a purity plate you should subculture the organism to a new plate so its pure and there are lots of colonies
how does preliminary reporting work for non sterile sites
-no prelims on day 2
-only final report on day 3 or a day 3 prelim report
What is done on day 3
-check all plates for additional growth -note NC
-make sure the ID makes sense
-read and report AST
-use Sus Reporting Guidelines to see what ABtics need to be reported out
-always read the footnote
What happens on Day 3 if your tests are mixed or if your control fails
-document that you would repeat the test
-dont report results for that test
-results would still be preliminary
What would day 3 be like for non sterile sites
could be normal flora depending on the body site
E.g. if viridans strep is isolated on a skin, it would be reported as ‘normal skin flora’
* If corynebacterium sp. was isolated in a sputum, it would be normal
respiratory flora.
