Body Fluids Flashcards

1
Q

What do the Lemellar body count, Phosphatidylglycerol (PG), and Lecithin-to-sphingomyelin ratio (L/S) tell us?

A

Evaluates amniotic fluid for fetal lung maturity.

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2
Q

Normal L/S ratio and ratio is fetal lung immaturity is implicated?

A

Normal: > or equal to 2

Fetal lung immaturity: < 2

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3
Q

What does ΔA450 tell us?

A

Amniotic fluid analysis determination for bilirubin to evaluate hemolytic disease of the newborn.

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4
Q

What does alpha fetoprotein (AFP) screen?

A

amniotic fluid analysis for neural tube defects/fetal development error

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5
Q

What does Acetylcholinesterase (AChE) used to screen?

A

Amniotic fluid analysis
Screen for neural tube defects (specific for this)

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6
Q

What is normal color and clarity of CSF?

A

Colorless and clear

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7
Q

Normal glucose in CSF?

A

50-80 mg/dl

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8
Q

When would you see elevated lactic acid in CSF?

A

Bacterial, TB, fungal causes of meningitis

Viral would be low

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9
Q

When would you see low lactic acid in CSF?

A

Viral meningitis

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10
Q

What could you do with the CSF of a multiple sclerosis person?

A

Protein electrophoresis of CSF for oligoclonal bands

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11
Q

Are white blood cells seen in CSF?

A

Yes, normally seen. (lymphocytes and monocytes)

NO RBCS SEEN

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12
Q

You would see this type of cell be increased in the CSF of someone who has MS.

A

Plasma cells

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13
Q

What is serous fluid?

A

Fluid that is the ultrafiltrate of plasma –> pleural, pericardial, peritoneal cavities

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14
Q

Serous Fluids
What is a transudate?

A

From systemic disease which is non-inflammatory.

Associated with congestive heart failure, hepatic cirrhosis, and nephrotic syndrome.

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15
Q

Serous Fluids
What is a exudate?

A

From inflammatory processes that increase permeability of the capillary endothelium in the parietal membrane.

Associated with infections, tumors, RA, and SLE.

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16
Q

What are chylous effusions?

A

Associated with damage to lymphatic system

When spun down, and is milky, presence of chyle –> presence of lymphocytes and chylomicrons (increase triglycerides)

17
Q

What is pseudochylous effusions?

A

From chronic inflammation (RA, TB)

No chylomicrons and low triglycerides, but present the same as chylous effusions.

18
Q

What is synovial fluid?

A

joint fluid

19
Q

How viscous is synovial fluid?

A

Highly viscous.

20
Q

What things do you look for in synovial fluid?

A

Crystals:
Monosodium urate crystals and Calcium pyrophosphate dihydrate crystals (CPPD) crystals

21
Q

What birefringence pattern would you see with monosodium urate crystals?

A

Negative birefringent; associated with gout

– yellow, parallel
– blue, perpendicular

22
Q

What birefringence pattern would you see with CPPD crystals?

A

Weakly positive birefringence; associated with pseudogout

– blue - parallel
– yellow – perpendicular

23
Q

Hemorrhage vs Traumatic Tap
Appearance of tubes?
Appearance of supernatant?
Presence of clots?

A

Hemorrhage: tubes all equally red, supernatant is xanthochromic, no clots

Traumatic Tap: subsequent clearing of blood in each tube, supernatant clear, clots due to fibrinogen

24
Q

What is normal color & liquefaction of seminal fluid?

A

Color- gray/white
Liquefaction: 30-60 min

25
Q

What is normal pH of seminal fluid?

A

7.2 - 7.8 (slightly basic)

26
Q

What is the normal viscosity of seminal fluid?

A

Watery – forms discrete droplets

27
Q

What is normal sperm motility?

A

> 50% motile with forward progression.

28
Q

What is normal agglutination in seminal fluid?

A

none

29
Q

What is normal sperm viability?

A

> 50% vital sperm (ASCP module)

Bottom line says >75%

30
Q

What is normal sperm concentration?

A

20-250 million / mL

(should see azoospermia [absence of sperm] 12 weeks post-vasectomy)

31
Q

For forensic studies, the presence of ___ confirms presence of semen in alleged rape cases.

A

Acid phosphatase

32
Q

In fecal samples, what two substances are used to screen for presence of activated neutrophils associated with inflammatory processes?

A

Lactoferrin and calprotectin

33
Q

What does the occult blood test inform us of?

A

Occult blood detects blood in feces that could be indicative of GI bleeding.

34
Q

What is normal sperm morphology?

A

Normal you want to aim for < 30% abnormal forms.

35
Q

What is the lactic acid values in transudate vs exudate?

A

Transudate: <200
Exudate >200

36
Q

What is the turbidity in transudate vs exudate?

A

Transudate: clear, watery
Exudate: cloudy, viscous (because its associated with infections and malignancies)