Blood vessels, blood haematopoiesis Flashcards
Aorta H&E
(1) Has 3 main layers
① Tunica intima
A. Endothelial Layer with Basal Lamina
B. Subendothelial Layer
(A) Loose connective tissue with some smooth muscle cells + collagen + Elastic fibers
(C) Internal Elastic Lamina
Found b/w tunica intima and tunica media
② Tunica media
A. Made up from 30-70 concentrically arranged
(A) elastic laminae
(B) smooth muscle cells
(C) some Circular Elastic Fibers, collagen fibers and fibrocytes (above 3 are Not developed in Veins)
(B) External Elastic Lamina
a. Found b/w tunica media and tunica adventitia
③ Tunica Adventitia
A. Outer layer
B. Composed of
(A) primarly of connective tissue fibers
(D) Vasa Vasorum
(E) Vascular Nerves (Peripheral Nerve) (above 5 are well developed in Veins)
Aorta resorcin-fuchsin
(1) Has 3 main layers
① Tunica intima
A. Endothelial Layer with Basal Lamina
B. Subendothelial Layer
(A) Loose connective tissue with some smooth muscle cells + collagen + Elastic fibers
(C) Internal Elastic Lamina
Found b/w tunica intima and tunica media
② Tunica media
A. Made up from 30-70 concentrically arranged
(A) elastic laminae
(B) smooth muscle cells
(C) some Circular Elastic Fibers, collagen fibers and fibrocytes (above 3 are Not developed in Veins)
(B) External Elastic Lamina
a. Found b/w tunica media and tunica adventitia
③ Tunica Adventitia
A. Outer layer
B. Composed of
(A) primarly of connective tissue fibers
(D) Vasa Vasorum
(E) Vascular Nerves (Peripheral Nerve) (above 5 are well developed in Veins)
Artery, vein (HE)
(1) Has 3 main layers
① Tunica intima
A. Endothelial Layer with Basal Lamina
B. Subendothelial Layer
(A) Loose connective tissue with some smooth muscle cells + collagen + Elastic fibers
(C) Internal Elastic Lamina
Found b/w tunica intima and tunica media
② Tunica media
A. Made up from 30-70 concentrically arranged
(A) elastic laminae
(B) smooth muscle cells
(C) some Circular Elastic Fibers, collagen fibers and fibrocytes (above 3 are Not developed in Veins)
(B) External Elastic Lamina
a. Found b/w tunica media and tunica adventitia
③ Tunica Adventitia
A. Outer layer
B. Composed of
(A) primarly of connective tissue fibers
(D) Vasa Vasorum
(E) Vascular Nerves (Peripheral Nerve) (above 5 are well developed in Veins)
Veins: Veins have thinner walls, larger diameter and greater structural variates veins contain valves duct to prvent back flow.
Artery, vein (HE)
(1) Has 3 main layers
① Tunica intima
A. Endothelial Layer with Basal Lamina
B. Subendothelial Layer
(A) Loose connective tissue with some smooth muscle cells + collagen + Elastic fibers
(C) Internal Elastic Lamina
Found b/w tunica intima and tunica media
② Tunica media
A. Made up from 30-70 concentrically arranged
(A) elastic laminae
(B) smooth muscle cells
(C) some Circular Elastic Fibers, collagen fibers and fibrocytes (above 3 are Not developed in Veins)
(B) External Elastic Lamina
a. Found b/w tunica media and tunica adventitia
③ Tunica Adventitia
A. Outer layer
B. Composed of
(A) primarly of connective tissue fibers
(D) Vasa Vasorum
(E) Vascular Nerves (Peripheral Nerve) (above 5 are well developed in Veins)
Veins: Veins have thinner walls, larger diameter and greater structural variates veins contain valves duct to prvent back flow.
Artery, vein orcein
(1) Has 3 main layers
① Tunica intima
A. Endothelial Layer with Basal Lamina
B. Subendothelial Layer
(A) Loose connective tissue with some smooth muscle cells + collagen + Elastic fibers
(C) Internal Elastic Lamina
Found b/w tunica intima and tunica media
② Tunica media
A. Made up from 30-70 concentrically arranged
(A) elastic laminae
(B) smooth muscle cells
(C) some Circular Elastic Fibers, collagen fibers and fibrocytes (above 3 are Not developed in Veins)
(B) External Elastic Lamina
a. Found b/w tunica media and tunica adventitia
③ Tunica Adventitia
A. Outer layer
B. Composed of
(A) primarly of connective tissue fibers
(D) Vasa Vasorum
(E) Vascular Nerves (Peripheral Nerve) (above 5 are well developed in Veins)
Veins: Veins have thinner walls, larger diameter and greater structural variates veins contain valves duct to prvent back flow.
Spermatic cord H&E
1. ductus deferens / vas deferens: mucosa
(1) mucous membrane:
① pseudostratified columnar epithelium with stereocilia:
A. stereociliated epithelial cell / principal cell
B. basal cell
② lamina propria
(2) muscular layer / muscular coat
① outermost longitudinal muscle layer
② middle circular muscle layer
③ innermost longitudinal muscle layer
The cremaster muscle can be found
Blood vessels can be found here as well.
Blood smear(MGG)
May-Grünwald –Giemsa
(a mixture of solutions of methylene
blue, eosin and azures)
Erythrocytes:
Small cells (7 to 8 µm diameter)
Biconcave disc
No nucleus
Platelets
Small discs (2 to 4 µm diameter)
No nucleus
Neutrophils
15 µm diameter
Segmented nucleus with 2 to 5 lobes
Cytoplasm is a pale blue-pink
Specific granules are pink-salmon
Few azurophilic granules
Eosinophils
12 to 15 µm diameter
Nucleus is bilobed
Cytoplasm is a pale blue-pink
Eosinophilic, specific granules - oval, 1 to 1.5 µm diameter, red/orange
Few azurophilic granules
Basophils
Smaller cells (10 to 12 µm diameter)
Nucleus bilobed or S-shaped
Cytoplasm is a pale blue-pink
Specific granules - 1 to 1.5 µm diameter,many very basophilic (blue to black)
Few azurophilic granules
Lymphocytes small and large
Round cells of 6 to 9 µm -9 to 15 µm diameter
Intense staining of the spherical nucleus of mostly condensed chromatin
Cytoplasm is limited to a small rim that is pale blue
No specific granules
Monocytes are the largest type of white blood cell.
Large cells (12 to 20 µm diameter)
Large, kidney-shaped or indented nucleus often with a “raked” chromatin pattern
Cytoplasm is pale blue
Few azurophilic granules
There are two types of bone marrow:
Red bone marrow - formation of the various types of blood cells (hematopoiesis). Red in color because of the hemoglobin in red blood cells (erythrocytes).
Yellow bone marrow - contains a high proportion of adipocytes
Megakaryocytes
A. Large bone marrow cell with a lobular shaped nucleus
(A) responsible for the production of blood thrombocytes (platelets)
- Red Bone Marrow
- Endochondral ossification (ossification zones):
(1) bony spicules (in sponge bone are) [trabeculae]
① are laid down in the formation of new bone matrix. - Skeletal muscle
- White (yellow) adipose tissue
- Brown fat tissue
- Periosteum
- Perichondrium
