Blood Stain Flashcards
Study of antigen antibody reaction used for purpose of law OR Study of body fluids OR Study of trace evidence
Forensic serology
State the Theory of Exchange, also called Locard’s principle of exchange.
Whenever two objects come in contact with each other, there is always some exchange of material from one to the other, which may not be visible to naked eye.
What is trace evidence?
Material evidence collected from the scene of crime that could be anything eg. Blood, semen, saliva, milk, urine, feces or any other material like hair, buttons etc.
Blood can be important evidence in
Murder
Injury
Sexual offences
Whether the stain is blood or some other material. What test to do?
Benizidine test
Phenolphthalein test
Kastle Mayer’s test
If benzidine test shows blue color then it is _________
Positive
If phenolphthalein test shows pink color then it is ________
Positive
What is the principle of Kastle Mayer’s test?
Hb detection test
What is luminol test?
A spray done to detect iron in the blood. Glow last for about 30 seconds and is blue in color.
What are the confirmatory test?
Microscopic test
Takayama’s test
Teichman’s test
Spectroscopy
If Adult Hb in spectroscopic test
D and E bands recognized
If fetal Hb in spectroscopic test then
Wide F bands along with D and E
Whether the blood is human or animal in origin, how to find out?
Precipitin test
Hemagglutination inhibition test
Latex test
Enzymological Test - LDH or Peroxidase (Px)
What is precipitin test?
Human blood has antigens so we inject them in animals like rabbit in smaller quantities for longer time, antibodies are developed in rabbit against human antigens. Later the rabbit serum and human blood sample is mixed and causes agglutination.
Wet bright red, starchy feel then whats the age of the stain?
Fresh
Red liquid blood age of stain?
half hour
Sticky feel blood the age of stain?
2 hours
Yellow liquid like blood so what is the age of stain?
3-8 hours