BLOOD SPECIMEN

Question 1

sometimes called Pre- analytical errors -
occur before the analytical

Answer 1

PRE EXAMINATION VARIABLE

Question 2

Pre-examination Errors that occur prior to
testing comprises _____% of all errors

Answer 2

46-56

Question 3

The most crucial and potentially
most dangerous pre-examination
error.

Answer 3

PATIENT IDENTIFICATION

Question 4

No longer than 1 minute
Ma’am: If longer than 1
minute it will result to
hemoconcentration and it
will lead to destruction of
RBC’s
3-4 inches above the
puncture site.
Increase the intravascular blood
pressure

Answer 4

TOURNIQUET APPLICATION

Question 5

reduction of fluid volume
inability to circulate lowers the pH
and causes potassium to be forced
out of blood cells.

Answer 5

Hemoconcentration

Question 6

Vigorous exercise INCREASES in
CK, AST, LDH, Bilirubin, creatinine,
and uric acid.
Lactate and Pyruvate - Increase in
moderate exercise due to the
increased metabolic activity of
skeletal muscle

Answer 6

EXERCISE POSTURE

Question 7

Collected at monitoring before
meal (NPO) Latin “Non Per
Orem” 8-12 hours no food - Fasting time

Answer 7

FASTING AND TIMED SAMPLES

Question 8

“Non Per
Orem”

Answer 8

“No food and
water intake”- Fasting

Question 9

Drawn at time intervals because of
medication or biological rhythms. EX. DIRUNAL EFFECT

Answer 9

TIMED SAMPLES

Question 10

Common diurnal, cortisol test: drawn
on a patient at _____ first extraction and
the second extraction

Answer 10

8 am; 4 pm

Question 11

Incorrect volume of blood
Less blood dilutional effect-RBCs
decreased in size
Too much blood clot
Partially filled tubes cannot be poured
together
Wrong anticoagulant

Answer 11

EVACUATED TUBES

Question 12

70% isopropyl alcohol
Povidone-Iodine (Betadine): for blood
culture collection ONLY- increase
potassium,phosphorus and uric acid

Answer 12

CLEANING VENIPUNCTURE SITE

Question 13

Collect order prevents the
anticoagulant form one tube from
contaminating the next tube

Answer 13

ORDER OF DRAW

Question 14

Mixture of ice and water as soon as
the blood is withdrawn

Answer 14

CHILLING OF SAMPLES

Question 15

Common tests requiring chilling of
specimens:

Answer 15

Ammonia, Acid
Phosphatase, Catecholamines, Lactic
Acid, pH/blood gasses

Question 16

SAMPLES Maintained at 37 degrees Celsius:

Answer 16

Cold
agglutinins, Cryoglobulin

Question 17

Maintained at body temperature
Wrap the sample in a heel warmer or
warmer block.

Answer 17

WARMING SAMPLES

Question 18

Bilirubin, Vitamin C, e, and K-
Amber Tube

Answer 18

EXPOSURE TO LIGHT

Question 19

The infectious substance which, when
exposure to it occurs, is capable of

causing permanent disability, life-
threatening or fatal disease in

otherwise healthy humans or
animals.

Answer 19

BIOLOGIC SUBSTANCE, Category A.

Question 20

An infectious substance that does
not meet the criteria for inclusion
in Category A.

Answer 20

BIOLOGIC SUBSTANCE, Category B.

Question 21

WHOLE BLOOD
- Maintain the specimen at _____________1
- Do not place whole blood
specimens in direct contact with
_____2.
- Do not ________3. if the sample
the requirement is whole blood.
4. STORAGE:
5. TEST: CRE

Answer 21

1. room
   temperature.
2. cool/ice
3. centrifuge
4. Ref Temp (48 hours)
5. CBC, Reticulocyte Count, ESR

Question 21

PLASMA/SERUM
1. Allow the sample to clot in an _____
position within _____ mins.
2. Centrifuge for at least ___ mins at
________ RPM
3. Separate the plasma/serum using a
______.
4. STORAGE: Ref temp: ____
Frozen temp: ______

5. TEST: CM, CT, CHEM, SERO

Answer 21

1. upright; 15-30
2. 15; 2200-2500 RPM
3. pipette
4. 48 hours; 1 year
5. Crossmatching,
   Coagulation Test, Chemistry
   Test, Serology Test