Blood Groups and Serologic Testing

Question 1

A description of the anti-globulin test is

Answer 1

D. AHG reacts with human globulin molecules bound to RBCs

Question 2

Polyspecific AHG reagent contains

Answer 2

Anti-IgG and Anti- C3d

Question 3

Monoclonal Anti C3d is:

Answer 3

A. Derived from one clone of plasma cells

Question 4

Which of the following is a clinically significant antibody whose detection has been reported in some instances to be dependent on anti complement activity in polyspecific AHG?

Answer 4

Anti-Jka

Question 5

After the addition of Igg coated RBCs (check cells) to a negative AHG reaction during an antibody screen, a negative result is observed:

Answer 5

The antibody screen cannot be Interpreted

Question 6

RBCs must be washed in saline at least three times before the addition of AHG reagent to:

Answer 6

B. Remove traces of free serum Globulin

Question 7

An in vivo phenomenon associated with a positive DAT is:

Answer 7

D. Maternal antibody coating fetal RBCs

Question 8

False-positive DAT results are most often associated with:

Answer 8

D. A positive autocontrol caused by polyagglutination

Question 9

Polyethylene glycol (PEG) enhances antigen antibody reactions by:

Answer 9

B. Concentrating antibody by removing water

Question 10

Solid-phase antibody screening is based on:

Answer 10

ADHERENCE

Question 11

Positive DAT may be found in:

Answer 11

HDFN

Question 12

What do coomb’s check cells consist of?

Answer 12

Type O positive cells coated with anti-IgG

Question 13

What method requires the use of check cells?

Answer 13

Manual Tube Method with albumin