Blood brain barriers with Lester Drewes

Question 1

3 tight junction proteins in the brain

Answer 1

Claudin

JAM (junctional adhesion molecule)

occludin

Question 2

What do Monocarboxylate transporters (like MCT1) in the brain do?

Answer 2

Transport fuels used when glucose in not around (lactate, pyruvate, acetate, butyrate = exercise, hypoglycemia)

(ketones = fasting, ketogenic diet)

Question 3

How much more is MCT1 expressed in neonates than adults?

people on ketogenic diets?

Answer 3

25 times more

8 times more

Question 4

What anti-cancer drug in class got into the brain via the “trogan horse” method?

What transporter does it use?

Answer 4

Paclitaxel

LRP - 1

Question 5

How do WBC’s get into the brain?

Answer 5

diapedesis;

Rolling, then emigration

Question 6

When does the BBB start to appear in humans?

Answer 6

First few weeks of fertilization.

Question 7

Glucose transporter most expressed in brain?

Answer 7

GLUT 1

Question 8

3 drugs that inhibit the formation of CSF and how they do it

Answer 8

Furosemide - inhibits Na, K, 2Cl transporter (NKCC transporter)
Ouabain - inhibits Na/K ATPase
Acetazolamide - inhibits cabonic anhydrase (CA)

Question 9

What is the glymphatic system and what does it do?

Answer 9

Garbage disposal of the brain.

The glymphatic system takes CSF and runs it into the paravenous system, where it clears it from the brain into the lymphatic system.