Blood Flashcards
Normal pH?
7.3 - 7.45
Plasma is an _ fluid
extracellular (ECF)
Blood comprises both
ECF and ICF (plasma and blood cells)
Blood helps maintain _
homeostasis
Fonctions of blood
- transport
- pH balance
- protection
Hematocrit
% of blood volume occupied by RBCs
Normal hematocrit
45%
Normal blood volume
normovolemia
Lower blood volume
hypovolemia
higher blood volume
hypervolemia
% of body mass in reference individual
7%
Liters in reference individual
5L
Composition is similar to
ISF
Blood mainly composed by
Water (more than 90%)
+ significant amount of ions
In very small concentration in blood (exchange at capillaries)
Nutrients, wastes, respiratory gases
Main difference between blood and ISF compositions
Proteins (colloids, 7g/dL) in blood
Percentage of colloids
Albumins 60%
Globulins 35%
Fibrinogen 5%
Blood composition may be approximated by
physiological saline (0.9g/dL NaCl)
Separation of plasma proteins by :
- differential precipitation by salts
- sedimentation in ultracentrafuge
- electrophoretic mobility
- immunological characteristics
What is electrophoresis?
Fractional method based on mvt of charged particles along a voltage gradient
(Apply current to a drop of plasma)
Rate of migration in electrophoresis influenced by:
- number of protein
- distribution of charges
- molecular weight of protein
