Blood Flashcards
Functions of blood
- Transport of dissolved gases, nutrients, hormones, salutes, ions and metabolic wastes
- Preserving acid base homeostasis
- Maintain body temperature. Distribute heat
- Clotting
- Immune functions
- Stabilize blood pressure - BV plays a role in determining this
Arterial stick
Artery collection of blood
Radial is common
-measure O2 level
Venipuncture
Collection of blood from vein
Median cubical
Why venipuncture vs arterial stick?
- Vein is thinner = easier to puncture
- Easy to locate, closer to surface
- Lower pressure - seals quicker than artery
WBC %
N, L, M, E, B
N (60-70) L (25-30) M (3-8) E (2-4) B (0.5-1)
Buffy coat
Platelets and leukocytes
Formed elements (RBC)
45%
4.8- 5.4 million
Platelets
150-400K
WBC count
5-10K
Physical characteristic
-8% body weight
-Volume: males: 5-6 L
females: 4-5 L
Ph: 7.35-7.45
Hypervolemic vs hypovolemic
Hyper is higher than normal
Hypo is lower than normal
Color: deoxygenated vs oxygenated
OXY - bright red (arterial)
DEOXY - dark red (venous)
Whole blood
When composition is unaltered
Serum
Plasma with clotting factors removed
Packed cell volume
% of blood comprised of formed elements 45% of blood volume (BV)
Hematocrit
% of blood compromised of RBC
Males: 40-54%
Females: 37-47%
Polycythemia
Abnormally high hematocrit
Makes blood viscous
Plasma %
55
Plasma composition
Water: 90-92% Plasma proteins: 7% - albumins, fibrinogens, globulins Solutes: 1% - electrolytes (Na, K, Mg, Cl, HCO, Ca) - glucose and amino acids - gases: O2 and CO2 - metabolic waste: urea, ammonia, bilirubin, uric acid, creatine - regulatory substances: hormones, enzymes and vitamins
Plasma proteins
90% made in liver
Too big to dissolve, remain in solution as colloid
(Albumins, globulins, fibrinogens)
Albumins
58%
Determines BCOP and regulates capillary exchange
Transports fatty acids and thyroid hormones
Globulins
38% Alpha: -protect against inflammation -transport thyroid and steroid hormones -coverts Fe2 -> Fe3 (take away ionic e-)
Beta:
- transferrin, transports iron
- beta lipoproteins, transports lipids
- complement proteins
Gamma:
-involved in immunity (antibodies)
Fibrinogen
4%
Clotting protein
Hematopoiesis
Formation of blood cells
- occurs in red bone marrow
- begins with hemocytoblasts
Hemacytoblast
Multi potent stem cell
Erythropoiesis vs leukopoiesis
Formation of both ‘cytes’
1st step of erythropoiesis
HEMOCYTOBLAST: can be any formed element
2nd step of hematopoiesis
ERYTHROCYTE - CFU: cell is committed to becoming an erythrocytes
3rd step of hematopoiesis
PROERYTHROBLAST: requires hormone erythropoietin
4th step of erythropoiesis
EARLY ERYTHROBLAST:
rapid synthesis of hemoglobin
5th step of erythropoiesis
LATE ERYTHROBLAST:
nucleus shrinks and is ejected with other organelles
6th step of erythropoiesis
RETICULOCYTE:
remaining organelles ejected, cell enters blood stream
7th step of erythropoeisis
ERYTHROCYTE:
mature cell now
Erythropoietin
Hormone that regulates RBC synthesis
-increases production in 2 ways
Increased RBC production:
- increased formation of proerythroblasts (more produced)
- increased rate of proerythroblast maturation