Block 1 exam

Question 1

ciInhibitors

Competitive “ CIty WiNe BAR MAKES FIne HeneSY”

Non Competitive “NIC LoVes All the RIBS & WiNe He Ate”

Irreversible “HAVe COAL”

Answer 1

comp= A weak non-covalent binder that binds the active site (a reversible reaction). Fixed levels of inhibitors allow

Question 2

Allosteric enzymes “EAt CrACkerS PAL”

Enzyme modification

“TRANSFER PoKemon Ash”

“HYPER DOg”

Answer 2

Allosteric enzymes use allosteric effectors to bind to the allosteric site; this causes conformational changes in the active site to bind a specific substrate

Positive/active effector shifts the graph left (lower Km but higher affinity
Negative/Inhibitory effector shifts it right (higher Km but lower affinity

Transferases (protein kinase) phosphorylates functional AA side chains (via ATP as a PO4- donor) to turn the enzyme ON

Hydroxylases (Phosphatases) dephosphorylate the functional AA side chains to turn the enzyme OFF

Question 3

Lygase/synthase “CAB”

Transferase “G-TRAAMP”

Hydrolase “PEG”

Oxidoreductase “OH DAM!”

Lyases/Synthetases “LAY tHe DEAD”

Answer 3

Lygase= Carboxylase (Biotin)

Transferase= Glycol-TR (UDP sugars), Acetyl-TR (Coenzyme A) Amino-TR (PLP, Vit B6, Pyroxidase), Methyl-TR (THF (folate) & SAM), Phospho-TR (ATP as PO4- donor)

Hydrolase= Peptidase (Protein), Esterase/lipase (fats), glucosidase/amilase (carbs)

Oxioreductase= Oxidase (heme), hydroxylase (NAD B3 Niacin, FAD B2 Riboflavin), Monooxygenase (Vit C. B3 Niacin)

Lyases= Hydroxylase/dehydroxylase, Deaminase, Decarboxylase (+PLP, Vit B6, pyridoxine)