Block 1 Genetics quiz 1 Flashcards
Genetic replication
initiation “Oh Her Sweet Titty SLP!”
Elongation “Real People Prefer Pears 5–>3 long lagging days Past Lunch”
Termination
Prereplication complex searches and binds to ORI —> Helicase unzips DNA at replication fork —> Single stranded binding proteins keep single strands from reannealing —-> Topoisomerase (I Eukaryotes, 2/DNA gyrase loosens supercoils via : snapping strands, loosening the coils, and patching the strands
RNA primase makes RNA primers (10-12 sequences long)—> DNA polymerase binds and synthesizes DNA in 5-to-3 direction —-> leading is continuous (1 primer) and the lagging is discontinuous (many primers) —> DNA pol 1 can excise incorrect pairs in the 5-3 direction and replace it with dNTP’s and replace the primers—> Ligase will glue the ends of the Okazaki fragments together
DNA polymerase run into Telomerase (TTAGGG)
Exogenous DNA damage “ER Regulars Can Develop Fever + Shakes”
Endogenous DNA damage “ Exposure to UV Dims Skin & makes me BRAF! Drink, Break, & Fuel”
Repeating sequences = Replication errors (issue with DNA polymerase, can’t recognize preparing sequence)
Chemical= Depurination (cleaved N-glycosidic bonds between deoxyribose and purines, free radicals, & spontaneous deamination (mismatching)
UV radiation: Formed Dimers (bulk DNA and stop replication and BRAF mutation —-> melanoma
Ionization= Double stranded breaks + free radicals
