Block 1 Flashcards
What is the normal range of WBC?
4.5 to 11 * 10^9/L
What levels of procalcitonin indicate bacterial infection?
0.5 to 2 = possibility, cant rule out infection
≥2 = bacterial infection
Empiric vs definitive
Educated guess
Empiric
Empiric vs definitive
Drug is aimed for specific “bug”
Definitive
Empiric vs definitive
Uses population data
Empiric
G+, cocci, catalase negative shape?
Chains
G+, cocci, catalase positive shape?
Clusters
G+, cocci, catalase +, coagulase +
S. aureus
G+, cocci, catalase +, coagulase -
S. epidermidis
S. saprophyticus
S. haemolyticus
G+, catalase -, alpha hemolysis
S. pneumoniae
S. mutans, S. sanguis
S. constellatus, S. intermedius, S. anginosus
G+, catalase -, beta hemolysis
S. constellatus, S. intermedius, S. anginosus
S. pyogenes (group A)
S. agalactiae (group B)
G+, catalase -, gamma hemolysis
Enterococci (E. faecalis, etc)
S. gallolyticus (group D)
G-, rods, lactose -, oxidase +
P. aeruginosa, C. jejuni, and Vibrio spp.
G-, rods, lactose -, oxidase -
Proteus, Shigella, and Salmonella
G-, rods, lactose +, oxidase -
Klebsiella
E. coli
Enterobacter
G+ or G-
Klebsiella
G-
G+ or G-
E. coli
G-
G+ or G-
Enterobacter
G-
G+ or G-
Shigella
G-
G+ or G-
Salmonella
G-
G+ or G-
Proteus
G-
G+ or G-
C. jejuni
G-
G+ or G-
P. aeruginosa
G-
G+ or G-
Vibrio spp.
G-
G+ or G-
Corynebacterium
G+
G+ or G-
L. monocytogenes
G+
G+ or G-
B. cereus
G+
G+ or G-
Propionibacterium
G+
G+ or G-
G. vaginalis
G+
G+ or G-
Lactobacillus
G+
G+ or G-
Clostridium
G+
G+ or G-
Erysipelotrix
G+
G+ or G-
Enterococci
G+
G+ or G-
S. gallolyticus
G+
G+ or G-
S. constellatus
G+
G+ or G-
S. pyogenes
G+
G+ or G-
S. agalactiae
G+
G+ or G-
S. pneumoniae
G+
G+ or G-
S. mutans
G+
G+ or G-
S. epidermidis
G+
G+ or G-
S. saprophyticus
G+
G+ or G-
S. haemolyticus
G+
G+ or G-
S. aureus
G+
Catalase + or -
S. aureus
+
Catalase + or -
S. epidermidis
+
Catalase + or -
S. saprophyticus
+
Catalase + or -
S. haemolyticus
+
Catalase + or -
S. pneumoniae
-
Catalase + or -
S. mutans
-
Catalase + or -
S. sanguis
-
Catalase + or -
S. intermedius
-
Catalase + or -
S. anginosus
-
Catalase + or -
S. constellatus
-
Catalase + or -
S. pyogenes
-
Catalase + or -
S. agalactiae
-
Catalase + or -
S. galloyticus
-
Catalase + or -
Enterococci
-
Catalase + or -
Erysipelotrix
-
Catalase + or -
Clostridium
-
Catalase + or -
Lactobacillus
-
Catalase + or -
G. vaginalis
-
Catalase + or -
Corynebacterium
+
Catalase + or -
L. monocytogenes
+
Catalase + or -
B. cereus
+
Catalase + or -
Propionibacterium
+
Coagulase + or -
S. aureus
+
Coagulase + or -
S. epidermidis
-
Coagulase + or -
S. saprophyticus
-
Coagulase + or -
S. haemolyticus
-
Alpha, Beta, or Gamma hemolytic
S. pneumoniae
Alpha
Alpha, Beta, or Gamma hemolytic
S. mutans
Alpha
Alpha, Beta, or Gamma hemolytic
S. sanguis
Alpha
Alpha, Beta, or Gamma hemolytic
S. consellatus
Alpha or Beta
Alpha, Beta, or Gamma hemolytic
S. intermedius
Alpha or Beta
Alpha, Beta, or Gamma hemolytic
S. anginosus
Alpha or Beta
Alpha, Beta, or Gamma hemolytic
S. pyogenes
Beta
Alpha, Beta, or Gamma hemolytic
S. agalactiae
Beta
Alpha, Beta, or Gamma hemolytic
Enterococci
Gamma
Alpha, Beta, or Gamma hemolytic
S. galloyticus
Gamma
Lactose + or -
P. aeruginosa
-
Lactose + or -
Proteus
-
Lactose + or -
Klebsiella
+
Lactose + or -
E. coli
+
Oxidase + or -
Klebsiella
-
Oxidase + or -
E. coli
-
Oxidase + or -
Proteus
-
Oxidase + or -
P. aeruginosa
+
Oxidase + or -
Enterobacteriacae
-
How long do cultures take?
3-5 days
Rapid diagnostic tests can take 15min to few hrs
What are the various rapid diagnostic tests available?
Rapid antigen test
PCR
MALDI-TOF
What is the gold standard antimicrobial susceptibility test?
Broth dilutions, however it is rarely done in practice