BIOTECHNOLOGY & ENVIRONMENTAL ISSUES Flashcards
using biological tools and knowledge for human purposes
biotechnology
manipulation of the genetic makeup of cells or whole organisms
genetic engineering
Type of genetic engineering wherein you recombine 2 DNA strands from 2 organisms for the purposes of making more and amplifying it
recombinant DNA technology
cuts DNA at specific cites
restriction enzymes
joins fragments of DNA
DNA Ligases
small circular pieces of DNA to which desired genes can be added and inserted to bacteria for amplification
plasmids
how do restriction enzymes know where to cut?
there are palindromic sequences and primase
machine assisted amplification of DNA
polymerase chain reaction
step 1 of PCR that heats the DNA to separate/denature the double helix into single strands
denaturation
cooling the reaction for 45 seconds so primers can bind to their complementary sequences on the single stranded template DNA
Annealing
heating for 2 minutes so that the TAQ polymerase extends the primers to know what to replicate as a partial whole
extension
final cooling at 5 C to ensure proper bonding of the newly synthesized DNA strand
cooling repeat cycle
used to know the sequence of ATCG
colorimetric experimentation
agar rose gel and electricity wherein you have negatively charged DNA samples that strain to positively charged power
Gel Electrophoresis
blood running through a machine, not often used because the suspects have to consent to give blood
blood strain
modifying plants for commercial purposes
transgenic plants
modified living organisms for the purpose of treatment and supplementation
transgenic organisms
generates more animal based products such as dairy
gene pharming
WHICH DISORDER:
- inherited lack of enzyme adenosine deaminase
- gene therapy introduces ADA to T cells and mitosis amplifies it
severe combined immunodeficiency (bubble boy)
WHICH DISORDER:
Over-accumulation of mucus and Stresses more than the respiratory system
Cystic Fibrosis
WHICH DISORDER:
over production of cells and using gene therapy by producing more interleukins
cancer
WHICH COVID PROTEIN
spikes attached to receptors of the human body
s-spikes
WHICH COVID PROTEIN
contains the infection
M-protein
WHICH COVID PROTEIN
releases the infection
E-protein
TRUE OR FALSE
- viruses contain RNA and DNA
FALSE - only RNA