Biotechnology Flashcards

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1
Q

Library Cloning

A
  • cDNA is cleaved and put in vectors
  • each vector has a different piece of DNA and these are put in bacteria
  • plated on petri plates
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2
Q

Why convert mRNA to cDNA

A

more stable

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3
Q

why use cDNA lib instead of mRNA

A

when you use cDNA you only look at expressed genes

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4
Q

how to convert RNA to cDNA

A
  • isolate the mRNA via poly-A-tail
  • reverse transcriptase uses mRNA as a primer to make DNA strand
  • Have an RNA-DNA hybrid
  • RNaseH cuts the RNA
  • DNA pol1 and DNA ligase synthesizes the second DNA strand
  • now there is DS DNA
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5
Q

Sanger Sequencing

A

-DS DNA molecule

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