Applied Biotechnology Flashcards
1
Q
CRISPR non-homologous end-joining
A
- once DNA is cut enzyme wants to prevent the drifting apart of the 2 DNA strands
- end binding proteins bind and crossbridge is formed
- gap repair and synthesis
2
Q
CRISPR Homologous Recombination Repair
A
donor dna is inserted in the cut. this is now expressed
3
Q
where do guide RNA and cas9 come from?
A
prokaryotes
4
Q
Amniocentesis
A
take cell from the amniotic fluid
culture cells and test for disease
if there is disease hard to abort cause it is late
5
Q
chorionic villus sampling
A
take cells from the fetus (chorionic villus)
test for disease
more invasive but faster
6
Q
Testing for Tay-Sachs Disease
A
in vitro fert
when hexA gene is present (good) it will fluoresce
this will then be implanted into mother
7
Q
Fluorescent In Situ Hybridization (FISH)
-changes in chromosome structure
A
- chromosome DNA denatured
- SS DNA probe with biotin is added
- fluorescent labelled avidin (binds to biotin is added)
- can now look thru microscope
8
Q
allele specific oligonucleotide testing
A
blue/white colour change
9
Q
Ashanti Desilva
A
- had severe combined immunodeficiency (SCID)
- non functional immune system
- WBC taken out and injected with enzyme adenosine deaminase (ADA)
- transferred back into blood stream. these replicated
