Biotechnology Flashcards

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1
Q

Define Biotechnology

A

Technology based on biology and involves the exploitation of living organisms

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2
Q

Name 4 ways that we use biotechnology

A

Production of foods
Production of drugs (Pharmaceutics)
Production of enzymes and chemicals
Bioremdiation of waste products

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3
Q

What occurs in the lag phase of bacteiral growth?

A

They become used to their surrondings

Slow reproduction

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4
Q

What occurs in the log phase of growth?

A

Populations breed and increase in size

Length of the phase = how quickly the species reproduce

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5
Q

Name four reasons why a plateau in population size may occur?

A

Lack of space
Conc of nutrients
Conc of toxic waste products
The carrying capacity is reached

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6
Q

Define Primary metabolites

A

Products of metabolism that are made during normal growth of an organism (eg, Amino acids / ethanol)

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7
Q

Define Secondary metabolites

A

Products of metabolism that are not produced as part of normal growth (eg, penecillin)

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8
Q

What type of conditions must fermentation be done under?

A

Sterile / aseptic

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9
Q

What is the main concern with continuous fermentation cultures?

A

Contamination can lose large volumes of product

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10
Q

Define Downstram processing

And what are the negatives of this?

A

The purification of the products of biotechnological reactions so that they can be used
Expensive
Needs lots of equipment

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11
Q

What are the four types of enzyme immobilization?

A

Adsorption
Covalent bonding
Entrapment
Membrane seperation

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12
Q

Explain adsorption (enzyme immobilisation)

A

Enzymes are attatched to a solid particle via weak bonds (eg ionic)
Many enzymes per particle
High probability of enzyme leakage

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