Biotechnology Flashcards

1
Q

Polymerase Chain Reaction(PMR)

A

A way of producing millions to billions of copies of a specific DNA sequence in just a few hours

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2
Q

Steps to (PMR)

A
  1. Separating- The Original DNA Strands
  2. Binding- Primers to each template strand
  3. Copying- The nucleotide sequence by adding nucleotide to template strand
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3
Q

Restriction Enzyme

A

A protein that cuts the DNA at specific DNA fragments

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4
Q

Gel Electrophoresis

A

A process that uses an electrical current to separate DNA fragments.

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5
Q

DNA Fingerprinting

A

A picture of the result from Gel Electrophoresis that identifies a person at the molecular level

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6
Q

Cloning

A

The process of creating a genetically identical copy of a gene or an organism

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7
Q

Steps to Cloning

A
  1. Remove diploid to haploid
  2. Insert Diploid nucleus into the egg cell
  3. Stimulate the egg cell with diploid nucleus to divide mitosis
  4. At the embryo stage it is placed into the surrogate mother
  5. A baby is born that looks like the original parent
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8
Q

Advantages to Cloning

A
  1. Increased food supply
  2. Saving endangered species
  3. Advance scientific discovery
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9
Q

Disadvantages to cloning

A

1.Success rate in mammals is low
2.Reduces Biodiversity 3.Expensive

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10
Q

Genetic Engineering

A

A technology that allows the DNA of a living cell to be modified

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11
Q

Recombination DNA

A

DNA that contains genes from two or more organisms

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12
Q

Genetically Modified Organism (GMO)

A

Organism that contain recombination DNA to provide a desired trait

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13
Q

Advantages to GMO’s

A
  1. Animals grow faster and larger
  2. Crops grow more efficiently and have a longer shelf life
  3. Disease and drought resistant
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14
Q

Disadvantages of GMO’s

A
  1. Little research has a long-term effect on health, but all research shows so far that there is no effect
  2. Introducing genetically altered bacteria to the environment with unknown consequence
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15
Q

Gene Knockout

A

A process that disrupts or turns off a gene ( when we do this, it gives us clues about the gene)

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16
Q

Gene Therapy

A

A technology that replaces a defective or missing gene to treat a disease, like cystic fibrosis

17
Q

Ethics

A

A system of moral principals that influence how we live