Biotechnology

Question 1

What is recombinant DNA?

Answer 1

a molecule of DNA that contains nucleotides from different sources

Question 2

What is exogenous DNA? Where is this DNA involved?

Answer 2

the DNA of another organism

involved in genetic engineering where DNA of one organism is manipulated in order to insert the DNA of another organism

Question 3

What are genetically engineered organisms used for?

Answer 3

1. study the expression of a particular gene within a species and between species
2. investigate cellular processes
3. study the development of a certain disease & discover new treatments

Question 4

How is a Gel electrophoresis prepared?

Answer 4

1. DNA is cut by a restriction enzyme
2. Fragments of various sizes are produced, and the DNA is negatively charged
3. Fragments move through an agarose gel medium towards the positive side
4. Smaller pieces travel farther & larger pieces travel slower and lag behind
5. A stain called ethidium bromide is applied to the DNA allowing the fragments to be visualized and the gel pattern analyzed

Question 5

What is Gel electrophoresis? Where is it used?

Answer 5

a method where an electric current is used to separate DNA fragments according to the size of the fragment

used to study DNA fragments various sizes; used to analyze DNA samples and create a DNA fingerprint for identification