BIOTECH PRINCIPLES

Question 1

for the preparation of the first recombinant DNA which organism was used as plasmid having the gene encoding for the antibiotic

Answer 1

Salmonella typhimurium

Question 2

the organism used as the host for the creation of 1st recombinant

Answer 2

e.coli

Question 3

the ppl to first create recomb. DNA

Answer 3

herbert boyer, stanely cohen

Question 4

what year was the 1st recomb. made

Answer 4

1972

Question 5

the first ren was

Answer 5

hind II

Question 6

during the naming of enzyme the first letter shows

Answer 6

genus

Question 7

middle 2 letters of enzyme name shows

Answer 7

species

Question 8

4 th letter shows

Answer 8

name of strain

Question 9

the roman numeral shows what

Answer 9

order in which the enzyme was isolated

Question 10

agarose gel is made from

Answer 10

sea weed

Question 11

compound used for staining of DNA

Answer 11

ethidium bromide

Question 12

amp. is used for recognising?

Answer 12

transformants

Question 13

the Prescence of chromogenic substrate gives which color i.e., donot have insert

Answer 13

blue

Question 14

a major pathogen in dicots are

Answer 14

agrobacterium tumefaciens

Question 15

the piece of DNA used by agrobacterium is called

Answer 15

T-DNA

Question 16

the virus that can cause cancer in animals is

Answer 16

retro virus

Question 17

the plasmid of agrobacterium which is tumor inducing is

Answer 17

Ti

Question 18

temp of heat shock is

Answer 18

42 c

Question 19

the bullets in gene gun have what metals

Answer 19

gold and tungsten

Question 20

DNA precipitates out on adding

Answer 20

chilled ethanol

Question 21

what kind of nucleotides are primers

Answer 21

oligo nucleotides

Question 22

thermostable nuclear polymerase

Answer 22

thermus aquaticus

Question 23

downstream processing includes

Answer 23

separation and purification