Biosynthesis and Degradation of Nucleotides Flashcards
What are the two parent nucleotides of nucleic acids?
adenosine 5’ monophosphate
guanosine 5’ monophosphate
de Novo pathways
“of the new” pathways tend to be longer and more complicated because the molecule is assembled from scratch with completely new parts
salvaged pathways
pathway that uses salvaged parts to create new molecules
PRPP
Phosphoribosyl pyrophosphate
What molecule does purine biosynthesis begin with?
PRPP
Phosphoribosyl pyrophosphate
What happens in the first step of the de novo purine synthesis pathway?
An amino group is donated by glutamine and this group is then attached to C-1 on PRPP
After the first step little groups are added one at a time to create what extremely unstable compound?
5-phosphoribosylamine
Unstable. Half life of 30s at pH of 7.5
In the purine molecule where does N-1 come from?
Aspartate
In the purine molecule where does C-2 and C-8 come from?
Formate
In the purine molecule where does N-3 and N-9 come from?
Amide N of glutamine
In the purine molecule where does the C-4 C-5 and N-7 come from?
glycine
In the purine molecule where does the C-6 come from?
Carbone Dioxide
What is the first intermediate in the de novo purine pathway?
Inosine monophosphate aka inosinate (IMP)
What is made from inosinate?
Adenosine monophosphate (AMP) Guanosine monophosphate (GMP)
What are pyrimidine molecules synthesized from?
What other two molecules are also made in this pathway?
aspartate PRPP and carbamoyl phosphate
Cytidine 5’ monophosphate
uridine 5’ monophosphate
