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PMLS Midterms > Biosafety and Security > Flashcards

Biosafety and Security Flashcards

1
Q

– Most important concept/ strict adherence
– Aware of potential hazard
– Trained and proficient in techniques
– Supervisors responsible for appropriate laboratory facilities, personnel and training

A

standard practices

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2
Q

any microbiological entity, cellular or non cellular, naturally occurring or engineered, capable of replication or of transferring genetic material that may be able to provoke infection, allergy, toxicity or other adverse effects in human, animals, or plants (eg. HIV, SARS-COV 2)

A

biological agent

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3
Q
  • any material comprised of, containing, or that may contain biological agents and/or harmful products such as toxins and allergens (eg. blood in test tube, sputum in the mask)
  • microorganisms that cause infection
A

biological material

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4
Q

potential source of harm caused by biological materials

A

biohazard

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5
Q

containment principles, technologies, and practices implemented to prevent unintentional / accidental exposure to pathogens and toxins, or their unintentional / accidental release

A

laboratory biosafety

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6
Q

institutional and personal security measures designed to prevent the loss, theft, misuse, diversion, or intentional release of pathogens and toxins

A

laboratory biosecurity

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7
Q

recapping of needles

A

biosafety concern

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8
Q

medtech is a member of terrorist group

A

biosecurity concern

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9
Q

lab personnel not provided with PPE

A

biosafety concern

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10
Q

disgruntled staff

A

biosecurity concern

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11
Q

work overload

A

biosafety concern

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12
Q

waste from the lab are not segregated

A

biosafety concern

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13
Q

lack of staff training

A

biosafety concern

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14
Q

staff with a huge dept

A

biosafety concern and biosecurity concern

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15
Q

most important concept and requires strict adherence

A

standard practices

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16
Q

responsible for appropriate laboratory facilities, personnel and training

A

supervisors

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17
Q

its purpose is to protect the laboratory worker

A

safety equipment

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18
Q
  • primary containment barrier
  • minimize exposure to hazard
A

safety equipment

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19
Q

engineering controls, equipment, PPE, Biosafety cabinet, Covered animal cage system, sealed centrifuge rotors are examples of which principles of biosafety?

A

safety equipment

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20
Q
  • second barrier
  • protects outside the laboratory
A

facility design and construction

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21
Q

Building and lab design, ventilation, autoclaves, cage wash facility, sealed laboratory walls/floors, HEPA filters are examples of which principles of biosafety?

A

facility design and construction

22
Q

biosafety levels are aka?

A

containment levels

23
Q

it is the combination of lab practices and procedures, safety equipment (primary barriers) and lab facilities (secondary barrier)

A

biosafety levels

24
Q
  • student training and teaching lab
  • no harm to humans
  • nonpathogenic organism
  • relies on standard microbiology practices
  • wear of PPE
A

biosafety level 1

25
Q

in this level of biosafety, standard microbiological practices are followed and work can be performed on an open lab bench or table

A

biosafety level 1

25
Q

facility construction
- a sink must be available for handwashing
- the lab should have doors to separate the working space with the rest of the facility

what level of biosafety is this?

A

biosafety level 1

26
Q
  • in this biosafety level, clinical, diagnostic, and teaching laboratories handling moderate risks agents
  • lab personnel should have specific training in handling pathogens
A

biosafety level 2

27
Q
  • access to the laboratory is restricted when work is being conducted
  • COMPLETE PPE is worn
  • all procedures that can cause infection from aerosols or splashes are performed within a biological safety cabinet (BSC)
  • An autoclave or an alternative method of decontamination is available for proper disposals
A

Biosafety level 2

28
Q

facility construction
- lab has self-closing doors
- a sink and eyewash is readily available

what level of biosafety is this?

A

biosafety level 2

29
Q
  • Builds upon the containment requirements of BSL-2
  • Clinical, research and production laboratories: Indigenous or exotic agents, Potential for respiratory transmission (Mycobacterium tuberculosis, St. Louis encephalitis, Coxiella)
  • Primary hazard: infectious aerosol
  • Emphasis on primary (protective clothing) and secondary barriers
  • Emphasis on procedures, must have written and validated Standard operating procedures
A

Biosafety Level 3

30
Q
  • Laboratorians are under medical surveillance and might receive immunizations for microbes they work with.
  • Access to the laboratory is restricted and controlled at all times
  • Safety equipment
  • Appropriate PPE must be worn, and respirators (N95) might be required.
  • All work with microbes must be performed within an appropriate BSC
A

BSL-3

31
Q
  • hands-free sink and eyewash
  • entrance to the lab: 2 sets of self-closing and locking doors
  • active security (cctv, id cards)
  • provides “zone of control” of hazards and odors
A

BSL-3

32
Q
  • dangerous/ exotic agents: via aerosol route, no vaccines or treatment, ebola and marburg viruses
  • extreme containment
  • maximum containment
A

BSL-4

33
Q
  • Change clothing before entering
  • shower upon exiting
  • decontaminate all materials before exiting
  • Class 3 Biosafety Cabinet
  • Full body, air supplied positive air pressure suits
A

BSL-4

34
Q
  • Separate buildings or completely isolated zone complex, specialized ventilation requirements
  • Special waste management
A

BSL-4

35
Q
  • Laboratories where work with microorganisms is conducted in a BSL2 lab with biosafety practices and procedures typically found in BSL3
    -Risk assessment based
  • Challenging since it is not a recognized level in biosafety guidance documents.
A

Biosafety Level 2+

36
Q
  • protection of sensitive sample from contaminant exposure
  • positive pressure areas
  • protection of personnel and the environment from biological exposure
  • negative pressure area
A

Biosafety Level 2+

37
Q
  • a microorganism that is unlikely to cause human/ animal disease
A

risk group 1

38
Q
  • a pathogen that can cause disease but is unlikely to be a serious hazard to lab workers, the community, livestock or the environment.
  • lab exposures may cause serious infection, but effective treatment and preventive measures are available and the risk of spread of infection is limited
A

risk group 2

39
Q

no or low individual and community risk

A

risk group 1

40
Q

moderate individual risk, low community risk

A

risk group 2

41
Q

high individual risk, low community risk

A

risk group 3

42
Q
  • a pathogen that usually causes serious disease but does not ordinarily spread from one infected individual to another
  • effective treatment and preventive measures are available
A

risk group 3

43
Q

high individual and community risk

A

risk group 4

44
Q

a pathogen that usually causes serious disease and that can be readily transmitted from one individual to another, directly or indirectly
- effective treatment and preventive measures are not usually available

A

risk group 4

45
Q
  • is the assurance of safety from physical intrusion
  • graded layers of protection
A

physical security

46
Q
  • assurance that the people are given access to sensitive biological materials should have that access
  • reduce the risk of theft or fraud, reduce scientific misconduct
A

personnel management

47
Q

assurance that there is an awareness of what exists in the lab, where it is and who is responsible for it

A

material control and accountability

48
Q
  • Protect biological materials in the laboratory and track those materials when they are transported outside of laboratory areas.
  • Utilize triple packaging for transporting biological materials.
  • Follow chain of custody principles and establish end-user agreements.
A

transport security

49
Q
  • Ensure that sensitive and valuable information stored in the laboratory is protected from theft or diversion.
  • Protect information that is too sensitive for public distribution.
  • communication and network security.
A

information security

50
Q

research that could be used for malevolent purposes

A

Dual Use research of concern (DURC)

51
Q

Monitor and assess the scientific, ethical, and social implications of certain biotechnologies. When necessary, monitor the development and integration of these technologies into scientific and clinical practice (e.g., gene editing, human gene transfer, recombinant technologies).

A

EMERGING BIOTECHNOLOGY

PMLS Midterms (5 decks)

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