Biosafety And Biosecurity

Question 1

Not known to consistently cause diseases in healthy adults

Answer 1

Biosafety Level 1 Agent

Question 2

Relatively common agents that are
associated with human disease

Answer 2

Biosafety Level 2 Agent

Question 3

Indigenous or exotic agents that may
cause serious or potentially lethal
disease through inhalation route of
exposure

Answer 3

Biosafety Level 3 Agent

Question 4

Dangerous or exotic agents which post high individual risk of aerosol- transmitted laboratory infections that are frequently fatal, for which there are

No vaccines or treatments

Answer 4

Biosafety Level 4 Agent

Question 5

Not associated with disease in healthy adult humans

No or low individual and community risk

Answer 5

Risk Group 1 Agents

Question 6

Associated with human disease that is rarely serious and for which preventive or therapeutic interventions are often available.

Moderate individual risk; low
community risk

Answer 6

Risk Group 2 Agents

Question 7

Associated with serious or lethal human disease for which preventive or therapeutic interventions may be available (high individual risk but low
community risk).

High individual risk; low community
risk

Answer 7

Risk Group 3 Agents

Question 8

_______ protects people from germs while ________ protects germs from people

Answer 8

Biosafety ;; Biosecurity

Question 9

Containment principles, technologies, and practices that are implemented to prevent unintentional exposure to pathogens or toxins, or their accidental release

Answer 9

Biosafety

Question 10

The protection, control, and accountability for valuable biological materials within the laboratories, in order to prevent their unauthorized access, loss, theft , misuse, diversion, or intentional release

Answer 10

Biosecurity

Question 11

Guidelines & regulations set by the Centers for Disease Control and Prevention (CDC) to prevent exposure to biohazards

Answer 11

Universal Precautions (UP)
Body Substance Isolation (BSI)
Standard Precautions (SP)

Question 12

Single most effective way of controlling the spread of infectious diseases

Answer 12

PROPER HAND WASHING

Question 13

Mandates that personnel should treat all blood and blood-contaminated
samples as potentially infectious

Answer 13

Universal Precautions (UP)

Question 14

Considers all body fluids and moist body substances to be potentially
infectious

Answer 14

Body Substance Isolation (BSI)

Question 15

Most commonly implemented by clinical laboratories

Combined UP and BSI

Answer 15

Standard Precautions (SP)

Question 16

Rubbing of hands should last for_____

Proper duration _______

Answer 16

20 sec

2 happy birthday song

Question 17

Protective clothing, helmets and other garments designed to protect the wearer’s body from injury or infection

Answer 17

PERSONAL PROTECTIVE EQUIPMENT (PPE)

Question 18

Device that encloses a workplace in such a way that protects the workers from exposure to aerosols that may potentially contain infectious disease agents

Answer 18

BIOSAFETY CABINETS (BSC)

Question 19

HEPA meaning

Answer 19

High Efficiency Particulate Air Filter

Question 20

HEPA filters

Pores having a diameter of _______

Removes air-suspended materials having diameter greater than ______

Capable of removing up to ______ of air-suspended materials

Answer 20

0.3 um

0.3 um

99.97%

Question 21

Also known as Open Front BSC

Answer 21

Biosafety Cabinet Class I

Question 22

Does not protect the sample/ product from possible contamination

Answer 22

Biosafety cabinet 1

Question 23

Protects the worker from potentially infectious aerosols; Also capable of
protecting the sample from possible contamination

Answer 23

Biosafety Cabinet Class II

Question 24

70% of air is recirculated to the working area; 30% of air is
exhausted

Answer 24

Types of BSC IIA

Question 25

30% of air is recirculated to the working area; 70% of air is exhausted

Answer 25

BSC Class IIB1

Question 26

No recirculation of air; Total exhaust of air through an exhaust HEPA filter

Answer 26

BSC Class IIB2

Question 27

Completely enclosed and are equipped with glove ports; Infectious material within is handled with rubber gloves that are attached and sealed

Answer 27

Biosafety Cabinet Class III

Question 28

Minimal Risk Practices include the essentials of Standard Precautions Does not utilize Biological Safety Cabinets, only needs hand washing facilities

Answer 28

Biosafety Level I (BSL-1)

Question 29

Vaccination against common infectious agents such as Hepatitis B Limiting access to the laboratory during working procedures Training laboratory personnel in handling pathogenic agents Direction by competent supervisors Performing aerosol-generating procedures in BSC Class I or BSC Class II

Answer 29

Biosafety Level II (BSL-2)

Question 30

High risk Controlled access Decontamination of all wastes Decontamination of laboratory clothing before laundering Performing aerosol-generating procedures in BSC Class I, Class II, or Class III

Answer 30

Biosafety Level III (BSL-3)

Question 31

Extreme risk Clothing change before entering Shower on exit Special protective clothing Requiring performance of procedures inside BSC Class III

Answer 31

Biosafety Level IV (BSL-4)