Biosafety Flashcards
What are the different hazards in the laboratory?
1 Biological 2 Chemical 3 Fire 4 Electrical 5 Compressed gas 6 Radioactive
Pierce-proof sharps container
PEP bottles
Bleach containers
Media containers
These may be used when splashes, sprays, or splatters of biohazardous materials are anticipated.
Face shields
Class D
Call local fire station
What is an example of secondary containment?
Facility
Examples of primary containment
Practices/techniques
Engineering controls
It is the most common material for gloves usd in the laboratory.
Latex
Purpose of practices/techniques
For risk assessment
For management
Cause of 1% of lab-associated infections
Improper Facility Design
A more durable and pierce proof glove material
Nitrile
Cause of 90% of lab-associated infections
Improper laboratory practices
How do you test impermeability of gloves?
1 Hold the glove tightly in the palm area of the glove
2 All fingers should inflate
3 No holes should be present
Mechanical systems
Electrical Plumbing Communications Lighting Gas Air Conditioning and Ventilation Vacuum
Components of biosafety
Primary containment
Secondary containment
It is a set of rules implemented to prevent misuse, theft, loss, and intentional release of biohazards.
Biosecurity
Cause of 9% of lab-associated infections
Containment Equipment Failure
What is the minimum eye protection required?
Safety glasses equipped with solid side shields
Disinfectant
70% ethyl alcohol
1:10 recently diluted bleach
Ethylene oxide
Who are susceptible to the hazards?
Students
Trained workers
Disinfectant for large spills
Hypochlorite
These are infectious agents or other hazardous biologic materials that present a risk or potential risk to the health of humans, animals, or the environment.
Biohazards
Examples of engineering controls
Personal Protective Equipment
Sinks
Biosafety cabinets
Locks
Considerations in selecting gloves
1 Type 2 Permeability 3 Cut and puncture resistance 4 Material compatibility 5 Use 6 Animal model 7 Comfort 8 Allergic potential
Selection of gloves
Hazards involved
Activity to be conducted
Class C
Dry chemical
What are biosafety rules?
Set of rules to follow in a laboratory setting that helps minimize all risks possible when handling biohazards and other hazards encountered in the laboratory.
Universal precaution
Blood and body fluids must be handled as if all were positive for HIV and other blood-borne pathogens.
Objective of general rules and regulations
To prevent and minimize hazards in the laboratory, such as acquired diseases, injuries, or infections
It encompasses the practices, procedures, and use of equipment needed to ensure adequate safety conditions in all facilities that work with potentially infectious microorganisms and other biological hazards.
Biosafety
Energized electrical equipment
Class C
Glove material used when working with animals
Kevlar
What are HEPA Respirators?
Face protectors that serve as an air filter against some pathogens,
Personal safety practices
1 Wear complete PPE
2 Keep long hair tied
3 Avoid wearing contact lenses
4 Wear safety glasses
5 Application of cosmetics is prohibited
6 Keep fingers, pencils, pens, or other materials out of the mouth
7 Marking pens used in the lab should stay in the lab
8 Always wear closed-toe footwear when in the laboratory
9 Eating, drinking, and smoking are prohibited
10 Horseplay and practical jokes are not allowed
11 Wounds, boils, or other open injuries must be properly protected from exposure
12 Always change PPE and clean up after handling and processing lab specimen
Flammable liquids
Class B
Contact time for a 100 ml blood spill
30 minutes
Safe laboratory practices
1 Each employee and laboratory personnel must be aware of the location and operation of all safety equipment and facilities in the laboratory
2 Never store food and drinks in laboratory refrigerators
3 Mouth pipetting is absolutely prohibited
4 Avoid patient contact when ill with skin infection, acute respiratory tract infections, or other contagious diseases
Administrative control
Includes employee education and orientation
Covers appropriate laboratory practices, including proper disposal of hazardous lab waste
Provision of post exposure plans and control
Sequence of wearing PPE
1 Hand hygiene 2 Lab gown 3 Masks/respirators 4 Goggles/Face shield 5 Gloves
Disinfectant for general cleaning
1% bleach
Examples of post-exposure plans and control
1 Immediate medical care for exposed individuals
2 Follow-up treatment
3 Proper accident investigation and assessment
4 Corrective action
Engineering Control/Laboratory Design
Mechanical systems
Air-handling systems
Appropriate display of biohazard symbols
Use of Biological Safety Cabinet
Sequence of removing PPE
1 Gloves 2 Lab gown 3 Hand hygiene 4 Eye protection 5 Masks 6 Hand hygiene
Class of BSC that allows room air to pass into the cabinet, as well as sterile exhausted air
Class I
Disinfectant for small spills
10% bleach
Process by which all forms of microbial life
Sterilization
Physical methods of sterilization
Incineration Moist heat Dry heat Filtration Ionizing radiation
Chain properly and store in well-ventilated areas
Tanks
Combustible metals
Class D
Chemical safety
Chemical hygiene plan and guidelines
Use of fume hoods
Use of PPE when handling toxic or noxious chemicals
Proper storage area for all chemicals
Class of BSC that is completely enclosed, negative pressure
Class III
Proper labeling includes:
Content Hazard warnings Special precautions Date received or prepared Date opened/put in use Expiration date Manufacturer's Material Safety Data Sheet
Class B
Carbon dioxide
Place in chained special dollies
Transport cylinders
Chemical methods of sterilization
Ethylene oxide
Formaldehyde vapour and vapour-phase hydrogen peroxide
Glutaraldehyde and peracetic acid
Ordinary combustible materials
Class A
Used for cold sterilization
Glutaraldehyde
Peracetic acid
Class A
Pressurized water
Class of BSC that allows sterilized air to flow over infectious material, as well as exhausted air
Class II
