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Clinical Microscopy > Biosafety > Flashcards

Biosafety Flashcards

0
Q

What are the different hazards in the laboratory?

A 
1 Biological
2 Chemical
3 Fire
4 Electrical
5 Compressed gas
6 Radioactive
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1
Q

Pierce-proof sharps container

A

PEP bottles
Bleach containers
Media containers

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2
Q

These may be used when splashes, sprays, or splatters of biohazardous materials are anticipated.

A

Face shields

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3
Q

Class D

A

Call local fire station

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4
Q

What is an example of secondary containment?

A

Facility

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5
Q

Examples of primary containment

A

Practices/techniques

Engineering controls

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6
Q

It is the most common material for gloves usd in the laboratory.

A

Latex

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7
Q

Purpose of practices/techniques

A

For risk assessment

For management

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8
Q

Cause of 1% of lab-associated infections

A

Improper Facility Design

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9
Q

A more durable and pierce proof glove material

A

Nitrile

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10
Q

Cause of 90% of lab-associated infections

A

Improper laboratory practices

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12
Q

How do you test impermeability of gloves?

A

1 Hold the glove tightly in the palm area of the glove
2 All fingers should inflate
3 No holes should be present

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13
Q

Mechanical systems

A 
Electrical
Plumbing
Communications
Lighting
Gas
Air Conditioning and Ventilation
Vacuum
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14
Q

Components of biosafety

A

Primary containment

Secondary containment

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15
Q

It is a set of rules implemented to prevent misuse, theft, loss, and intentional release of biohazards.

A

Biosecurity

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15
Q

Cause of 9% of lab-associated infections

A

Containment Equipment Failure

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17
Q

What is the minimum eye protection required?

A

Safety glasses equipped with solid side shields

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18
Q

Disinfectant

A

70% ethyl alcohol
1:10 recently diluted bleach
Ethylene oxide

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19
Q

Who are susceptible to the hazards?

A

Students

Trained workers

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22
Q

Disinfectant for large spills

A

Hypochlorite

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23
Q

These are infectious agents or other hazardous biologic materials that present a risk or potential risk to the health of humans, animals, or the environment.

A

Biohazards

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23
Q

Examples of engineering controls

A

Personal Protective Equipment
Sinks
Biosafety cabinets
Locks

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23
Q

Considerations in selecting gloves

A 
1 Type
2 Permeability
3 Cut and puncture resistance
4 Material compatibility
5 Use
6 Animal model
7 Comfort
8 Allergic potential
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25
Q

Selection of gloves

A

Hazards involved

Activity to be conducted

26
Q

Class C

A

Dry chemical

27
Q

What are biosafety rules?

A

Set of rules to follow in a laboratory setting that helps minimize all risks possible when handling biohazards and other hazards encountered in the laboratory.

28
Q

Universal precaution

A

Blood and body fluids must be handled as if all were positive for HIV and other blood-borne pathogens.

29
Q

Objective of general rules and regulations

A

To prevent and minimize hazards in the laboratory, such as acquired diseases, injuries, or infections

30
Q

It encompasses the practices, procedures, and use of equipment needed to ensure adequate safety conditions in all facilities that work with potentially infectious microorganisms and other biological hazards.

A

Biosafety

31
Q

Energized electrical equipment

A

Class C

32
Q

Glove material used when working with animals

A

Kevlar

32
Q

What are HEPA Respirators?

A

Face protectors that serve as an air filter against some pathogens,

33
Q

Personal safety practices

A

1 Wear complete PPE
2 Keep long hair tied
3 Avoid wearing contact lenses
4 Wear safety glasses
5 Application of cosmetics is prohibited
6 Keep fingers, pencils, pens, or other materials out of the mouth
7 Marking pens used in the lab should stay in the lab
8 Always wear closed-toe footwear when in the laboratory
9 Eating, drinking, and smoking are prohibited
10 Horseplay and practical jokes are not allowed
11 Wounds, boils, or other open injuries must be properly protected from exposure
12 Always change PPE and clean up after handling and processing lab specimen

35
Q

Flammable liquids

A

Class B

36
Q

Contact time for a 100 ml blood spill

A

30 minutes

38
Q

Safe laboratory practices

A

1 Each employee and laboratory personnel must be aware of the location and operation of all safety equipment and facilities in the laboratory
2 Never store food and drinks in laboratory refrigerators
3 Mouth pipetting is absolutely prohibited
4 Avoid patient contact when ill with skin infection, acute respiratory tract infections, or other contagious diseases

39
Q

Administrative control

A

Includes employee education and orientation
Covers appropriate laboratory practices, including proper disposal of hazardous lab waste
Provision of post exposure plans and control

40
Q

Sequence of wearing PPE

A 
1 Hand hygiene
2 Lab gown
3 Masks/respirators
4 Goggles/Face shield
5 Gloves
40
Q

Disinfectant for general cleaning

A

1% bleach

40
Q

Examples of post-exposure plans and control

A

1 Immediate medical care for exposed individuals
2 Follow-up treatment
3 Proper accident investigation and assessment
4 Corrective action

41
Q

Engineering Control/Laboratory Design

A

Mechanical systems
Air-handling systems
Appropriate display of biohazard symbols
Use of Biological Safety Cabinet

42
Q

Sequence of removing PPE

A 
1 Gloves
2 Lab gown
3 Hand hygiene
4 Eye protection
5 Masks
6 Hand hygiene
42
Q

Class of BSC that allows room air to pass into the cabinet, as well as sterile exhausted air

A

Class I

43
Q

Disinfectant for small spills

A

10% bleach

46
Q

Process by which all forms of microbial life

A

Sterilization

46
Q

Physical methods of sterilization

A 
Incineration
Moist heat
Dry heat
Filtration
Ionizing radiation
47
Q

Chain properly and store in well-ventilated areas

A

Tanks

48
Q

Combustible metals

A

Class D

49
Q

Chemical safety

A

Chemical hygiene plan and guidelines
Use of fume hoods
Use of PPE when handling toxic or noxious chemicals
Proper storage area for all chemicals

50
Q

Class of BSC that is completely enclosed, negative pressure

A

Class III

52
Q

Proper labeling includes:

A 
Content
Hazard warnings
Special precautions
Date received or prepared
Date opened/put in use
Expiration date
Manufacturer's Material Safety Data Sheet
53
Q

Class B

A

Carbon dioxide

54
Q

Place in chained special dollies

A

Transport cylinders

55
Q

Chemical methods of sterilization

A

Ethylene oxide
Formaldehyde vapour and vapour-phase hydrogen peroxide
Glutaraldehyde and peracetic acid

55
Q

Ordinary combustible materials

A

Class A

56
Q

Used for cold sterilization

A

Glutaraldehyde

Peracetic acid

56
Q

Class A

A

Pressurized water

57
Q

Class of BSC that allows sterilized air to flow over infectious material, as well as exhausted air

A

Class II

Clinical Microscopy (3 decks)

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