Bioprocessing Flashcards
1
Q
What is bioprocessing?
A
the application of biotechnology on an industrial scale for processing substrates to desired products
2
Q
What are examples of Biopharmaceutical products?
A
- Proteins
- Recombinant Vaccines and Enzymes
- Cell and Gene Therapies
- Acellular Products
3
Q
What is Proinsulin?
A
- the inactive form of insulin
- it is a single polypeptide chain containing: A-chain, B-chain, C connecting peptide
4
Q
Describe the conversion to insulin
A
- Beta cells produce proinsulin
- Beta cell processes the proinsulin by the enzymatic removal of the C-peptide structure
- The resulting insulin is packaged and secreted by the cells
5
Q
What is the FULL PROCESS for insulin production from genetically engineering E. coli?
A
- Cultivate genetically engineered E. coli in bioreactors
- Harvest E. coli cells and obtain inclusion bodies
- Solubilize inclusion bodies
- CNBr Cleavage: converts recombinant pr- to Trp
- Sulfitolysis
- Ion exchange chromatography
- Refolding
- Enzymatic conversion
- Final Purification
6
Q
What is the shortened order of insulin production from GE E. coli?
A
- Cultivating genetically engineered E. coli in bioreactors
- Harvesting genetically engineering E. coli cells
- Solubilizing inclusion bodies
- Converting inclusion bodies to insulin
