Biology- required practical

Question 1

What is the method for the effects on the rate of reaction of Amylase?

Answer 1

Method:
1. Label a test tube with the first value of pH buffer
solution (pH 4)
2. Into each well of the spotting tiles, place a drop of iodine.
3. Using a measuring cylinder, measure 2cm³of amylase and pour into the
test tube.
4. Using a syringe, measure 1cm³ of the buffer solution and pour into the
test tube.
5. Leave this to stand for five minutes and then use the thermometer to
measureand record the temperature.
6. Add 2cm³ of starch solution into the test tube, using a measuring
cylinder to measure, and begin a timer
7. After 10 seconds, use a pipette to extract some of the amylase/starch solution,
and place one drop into the first well of the spotting tile.
8. Continue to place one drop into the next well of the spotting tile, every 10
seconds, until the iodine remains orange.
9. Record the time taken for the starch to be completely digested by the amylase
by counting the wells that were tested positive for starch . Each well represents 10 seconds of
time.
10. Repeat steps 1 to 8 for pH values 7 and 10.

Question 2

What is the order of the principles of organisation

Answer 2

smallest-1.cell 2.tissue 3.organ 4.organ system 5.organism