Biology Flashcards

1
Q

The Association of American Feed Control Officials (AAFCO) nutrient profiles are based on what?

A

The NRC of the United States National Academy of Sciences.

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2
Q

What is AAFCO? What does it provide?

A

An advisory body comprising state representatives from across the US. Provides mechanism for developing and implementing uniform and equitable laws, regulations, standards, and enforcement policies, and establishes nutrient profiles for dog and cat foods.

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3
Q

What is the maintenance energy requirement (MER)? How can it be calculated?

A

The amount of energy used by a moderately active adult dog in a thermoneutral environment. MEW = BW (kg) x 0.75 x 550 kJ digestible energy

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4
Q

What is an open formula diet?

A

Defined concentrations of all ingredients and the information is publicly available. Changes in formulation to maintain nutrient composition or meet changing requirements are made open.

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5
Q

What is a closed formula diet?

A

Meet and label the minimum requirements for protein and fat and the maximum volume for ash and fiber, but exact composition of ingredients may vary from batch to batch. Manufacturer applies least cost strategy by formulating diets to maximize profit by using least expensive ingredients. Ingredients are listed but quantitative ingredient formula not publicly available.

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6
Q

What is a fixed-formula diet?

A

Quantitative ingredient formulation does not change, but this information is proprietary and not publicly disclosed.

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7
Q

Describe semi-purified and purified diets.

A

Formulated from purified components: amino acids, lipids, carbs, vitamins, and minerals. Terms generally interchangeable. Purified-ingredient diets open formula.

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8
Q

As a general rule, how long are diets safe for consumption following the manufacture date when stored at room temperature?

A

6 months

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9
Q

Beagles at what age have the best conception rate and litter size with lowest neonatal mortality? At what age do conception rats and litter size decline and neonatal mortality increase?

A

Ideal between 2 and 3.5 years. Decline after 5 years old.

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10
Q

Approximately where is the cervix in relation to the ovaries and vulva? When can be it palpated in the abdomen?

A

Halfway between ovaries and vulva. Palpated in the abdomen in proestrus and estrus.

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11
Q

Describe general reproductive characteristics of female dogs.

A

Monoestrous, nonseasonal, spontaneous ovulators. Spontaneous luteal phase 5 days longer than the 65 +/- 1 days of pregnancy followed by obligate anestrus.

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12
Q

When does female puberty occur? What is it positively correlated with?

A

6-14 months. Positively correlated with body size.

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13
Q

What are the four stages of the canine estrus cycle?

A

Proestrus, estrus, diestrus, and anestrus.

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14
Q

How long does proestrus last? What hormonal and physical changes take place?

A

5-20 days, average of 9. Follicular phase rise in estrogen. Vulva enlarged and turgid, serosanguinous vaginal discharge present.

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15
Q

How long does estrus last? What are the endocrine features? What are physical characteristics?

A

5-15 days, averages 9 days. First abrupt increase in progesterone which occurs concomitantly with the LH surge, followed by ovulation in 24-72 hours. Vulva softer and smaller, vaginal discharge persists and may remain serosanguinous or become straw-colored.

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16
Q

When does diestrus begin? How long does it last? How is it behaviorally defined? Which hormone peaks?

A

Begins 9 days after onset of standing heat. Ends 60 days later, which would coincide with whelping if pregnancy occurred. Defined behaviorally as starting when estrous behavior ceases. Represents peak progesterone.

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17
Q

How long is anestrous? How is it characterized?

A

80-240 days. Stage of repro quiescence with an absence of ovarian activity and serum progesterone levels less than 1 ng/mL.

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18
Q

When does male puberty occur? How is this process initiated? Describe the hormonal pathway.

A

5 to 12 months. Initiated by secretion of LH from anterior pituitary, which stimulates the production of testosterone by the interstitial/Leydig cells. Testicular growth is rapid, seminiferous tubules begin to differentiate, and Sertoli cells form blood-testis barrier. Secretion of FSH by anterior pituitary stimulates Sertoli cells to produce inhibin, androgen binding protein, and estrogen. FSH stimulates spermatogenesis in presence of testosterone, inhibin and estrogen provide negative feedback to pituitary to decrease FSH production.

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19
Q

How long does spermatogenesis take? How long must sperm mature for in the epididymis? Thus, the entire process from initiation of spermatogonial mitosis to the delivery of mature sperm to the ejaculate takes how long?

A

45 days, 15 days, 60 days total.

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20
Q

What can cause suppression of sexual behavior and problems with libido in male dogs?

A

Early weaning, isolation, or inherited abnormalities.

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21
Q

What correlation exists between scrotum circumference and number of sperm produced?

A

Positive correlation

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22
Q

What characteristics of ejaculate are good indicators of normal male fertility?

A

Ejaculate (5 mL) with approx 500 million progressively motile sperm without sig. morphological abnormalities.

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23
Q

What occurs to the vaginal epithelium due to estrogen? When does this peak?

A

Increase in the percentage of vaginal epithelial cell cornification. Peaks 2 days prior to estrogen peak and 4 days prior to standing heat.

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24
Q

When does vaginal cytology of the bitch change from predominantly cornified to noncornified? What does this coincide with?

A

6 days after ovulation. Coincides with first day of diestrus.

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25
Q

What cells other than cornified cells are appreciated on vaginal cytology?

A

Superficial cells - Large, angular cells with small nuclei
Intermediate cells - Round or oval cells with abundant cytoplasm and large, vesicular nuclei
Parabasal cells - Small, round or elongated cells with large, well-stained nuclei and a higher nuclear/cytoplasm ratio.

26
Q

Based on vaginal cytology, how is the estrous cycle classified?

A

Proestrus, early - Intermediate and superficial cells, RBC, neutrophils
Proestrus, late - Superficial cells, anuclear squames, RBC
Estrus - More than 50% anuclear squames, superficial cells, +/- RBC
Diestrus - More than 50% intermediate cells, superficial cells, and squames early, but becoming completely noncornified with neutrophisl present as diestrus proceeds
Anestrus - Small number of parabasal cells and intermediate cells, +/- neutrophils

27
Q

What are behavioral indicators of the estrus cycle?

A

Proestrus - Male is attracted and will investigate her hindquarters but she will not accept breeding
Estrus - Proactive receptivity to mounting by males and increased male-seeking behavior. Flagging of tail

28
Q

To maximize conception rate and litter size, when should the female be bred?

A

Days 1, 3, and 5 of standing heat

29
Q

When does fertilization occur?

A

In oviduct up to 8 days after coitus.

30
Q

The ovulated oocyte is a primary oocyte that must undergo what before fertilization can occur? How long does the overall maturation process take? After maturation, how long is the oocyte viable for?

A

Two meiotic divisions. Maturation takes approx 2 days. Oocyte viable for 4-5 days.

31
Q

Breeding on what day results in optimal conception rates? Breeding on what day results in best litter size?

A

Bitch bred from 4 days before to 3 days after ovulation. Bred 2 days after ovulation.

32
Q

When does implantation occur? How is it evident?

A

17-18 days after breeding, Evident by areas of local endometrial edema.

33
Q

Is there a correlation between the number of corpora lutea and number of fetuses in the corresponding uterine horn?

A

No, suggests transuterine migration of embryos

34
Q

What type of placentation does the dog have?

A

Endotheliochorial placentation. Endothelium of uterine vessels lies adjacent to the fetal chorion, mesenchymal, and endothelial tissues, so maternal and fetal blood are separated by four layers.
Zonary, with placental villi arranged in a belt
Deciduate, maternal decidual cells are shed with fetal placentas at parturition.

35
Q

What is the length of gestation?

A

59-63 days.

36
Q

What hormone and structure is responsible for maintaining pregnancy?

A

Luteal progesterone produced by corpora lutea.

37
Q

What progesterone levels are found throughout estrus and pregnancy? What is progesterone important for?

A

Progesterone less than 1 ng/mL in late proestrus, peak of 30-60 ng/mL during gestation, and declines to 4-5 ng/mL just prior to parturition. Progesterone essential for endometrial gland growth, secretion of uterine milk, attachment of placentas, and inhibition of uterine motility.

38
Q

When can pregnancy be identified by palpation?

A

Most information approx 28 days after breeding. Embryos and chorioallantoic vesicles form series of ovoid swellings and are 2in in length at 28-30 days. Uterus enlarges diffusely by day 35.

39
Q

When is radiology useful in pregnancy determination?

A

45 days after LH surge.

40
Q

When can ultrasound confirm pregnancy? Assess fetal heartbeats? Assess fetal movements?

A

18-22, 23-25, and 35 days.

41
Q

How can ultrasound be used to estimate gestational age? When is it most accurate? What needs to be corrected for?

A

Use inner diameter of chorionic cavity early in pregnancy and biparietal diameter in late pregnancy. Most accurate at day 30 when accounting for small <9kg and large >40kg dogs.

42
Q

Why are shavings discourage for whelping dogs?

A

May adhere to umbilical cord and predispose to ascending infection.

43
Q

When can heat lamps be placed? What must be monitored?

A

24 hr prior to parturition until all neonates demonstrate vigorous suckling behavior. Watch for thermal burns.

44
Q

What indicates an impending parturition within 18-24 hours?

A

Abrupt drop of body temperature to less than 100 F.

45
Q

What occurs during the three stages of parturition?

A

Stage 1 - 6-12 hours. Uterine contractions and cervical dilation. Bitch restless, nervous, and anorexic. Panting and increased pulse
Stage 2 - 3-6 hours. Fetal expulsion. Ferguson reflex - Fetus engages cervix, results in release of oxytocin, which strengthens uterine contractions and may result in abdominal contractions. Bitch can inhibit this stage of labor if disturbed. Chorioallantois ruptures during passage of neonate through birth canal of bitch’s teeth. Posterior presentation common, does not predispose to dystocia. Irregular intervals between pups, average less than 1 hour. Assistance if in stage 2 for more than 5 hours without delivering the first pup or more than 2 hours before delivering subsequent pups.
Stage 3 - Placentas expelled immediately or within 15 min of delivery of each pup. Bitch licks newborn vigorously. May ingest placenta which can lead to transient diarrhea.

46
Q

Peripartum use of oxytocin is required only in what situations? When should it not be used? What are they indications for its use? What is recommended first?

A

Uterine inertia, stillbirths, of agalactia. Should not be used in event of systemic illness or abnormalities precluding vaginal delivery. Used if lack of delivery 24 hours after onset of stage 1 labor, greater than 1 hour of unproductive stage 2 labor, inadequate contractions, or abnormal vaginal discharge. Radiographs recommended to assess fetal size in relation to birth canal and any possible obstructions.

47
Q

How many doses of oxytocin may be given? What other agents may be indicated?

A

Total of two doses. Calcium gluconate and dextrose.

48
Q

When does uterine involution occur? What is considered normal during this time? What is concerning for metritis or pyometra?

A

During anestrus, within 4-5 weeks of parturition. Greenish to red-brown vaginal discharge, or lochia, is normal. Presence of odiferous, purulent discharge accompanied by systemic signs of illness indicates metritis or pyometra.

49
Q

When does desquamation of the endometrium begin and finish?

A

Begins 6th postpartum week, complete repair by 3 months.

50
Q

When do puppy eyes and ears open? When can solid food be introduced? When can puppies be weaned?

A

Eyes: 12 days
Ears: 12-20 days
Solid food: 4.6-6 weeks
Wean: 6-8 weeks

51
Q

When is AI indicated?

A

Male is physically incapable of mounting or penetrating bitch.
Vaginal abnormalities
Behavioral incompatability

52
Q

What volume of semen is collected for AI?

A

4-6 mL

53
Q

How can semen be introduced via AI?

A

Into cranial vagina or directly into uterus with trans-cervical catheterization with a Norwegian AI catheter of utilizing fiberoptic endoscopy.

54
Q

What is an advantage of use of the Norwegian AI catheter for intrauterine insemination of semen?

A

Higher whelping rates than intravaginal insemination

55
Q

When can AI with freshly collected sperm be done?

A

On days 1, 3, and 5 of standing heat or on days of max vaginal cornification.

56
Q

How does the viability of frozen-thawed sperm compared to fresh or chilled sperm? Therefore, what must occur?

A

Sig. reduced. Only lives a few hours in the bitch repro. tract. Ova must be mature and insemination must be done 2-3 days after ovulation as determined by serum progesterone.

57
Q

What is pseudocyesis? When does it occur?

A

False pregnancy, a stage of mammary gland development and lactation associated with nesting or mothering behavior. Occurs after the decline in serum progesterone towards the end of diestrus.

58
Q

Is there a breed or age predisposition? Does it predispose the bitch to reproductive disease or infertility?

A

No and no.

59
Q

When is reproductive behavior of the bitch optimal? When does sig. decrease in conception rate and number of live pups occur? What occurs by 8-9 years?

A

Optimal prior to 4 years. Cycling does not completely cease, but poorer performance after 5-8 years. By 8-9 years, pathologic conditions of uterus such as cysts, hyperplasia, atrophy, and neoplasia common.

60
Q

When are dogs socially mature? When should the socialization period start?

A

18-36 months of age. Socialization process should occur early in development.
3-8 weeks: Most capable of learning how to interact with other dogs
5-12 weeks: How to interact with people
10-12 weeks: Voluntarily wander and explore new environments.