Biology Flashcards
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Specimen
Object being examined under microscope.
Light Microscope
Uses light to magnify a specimen.
Electron microscope
Uses electrons to magnify a specimen.
What does the arm/ handle of the microscope do?
Connects the eyepiece down to the base of the microscope.
What does the coarse focus of the microscope do?
Controls focus quickly to get the specimen into focus.
What does the fine focus of the microscope do?
Makes fine adjustments to obtain a sharper image.
What does the stage on the microscope do?
It is where you place the slide.
What does the objective lens do?
Magnifies the image of the specimen (4x, 10x, 40x).
What is the eyepiece/ ocular lens of the microscope?
Magnifies image produced by the objective lens (10x).
What does the condenser do?
Focuses light onto a specimen.
How do you find out the total magnification?
To find out the total magnification, you must times the eyepiece/ ocular lens magnification by the objective lens magnification. (For example, 10x eyepiece magnification and 40x objective lens magnification would equal 400x total magnification because you times the 10 by the 40).
What is meant by the field of view or FOV of the microscope?
The FOV is the width of the circle you see when you are looking through a microscope.
What is meant by the resolution when looking through a microscope?
The resolution is the focus/ clarity of an image.
What happens to the FOV if the magnification gets greater?
When the magnification gets greater, the FOV gets smaller.
How do you calculate/ estimate the average size of one cell?
First, calculate the total magnification. Then look at the FOV and figure out how many cells fit into the width of the FOV. Then divide the FOV width by the number of cells (For example, if the FOV was 2500 micrometres and 4 cells could fit into the FOV, then divide the 2500 by the 4 (2500/4). That would mean the average size of one cell in that scenario would be 625 micrometres.
