Biological Safety Cabinets Flashcards
What is this?
What protections does it provide?
What is the standard inward air flow velocity?
What is required if toxic vapors are to be used?
Class I BSC
Provides personnel and environmental protection, but no product protection.
Personnel protection is maintained as long as inward flow velocity is at least 75 linear feet per minute (lfm) through front opening.
Must be hard ducted into building exhaust if toxic vapors are used.
What is this?
What protections does it provide?
What is the minimum inward air flow velocity?
How much air is recirculated?
What are limitations for its use?
Class II Type A1 BSC
Provides personnel, environmental, and product protection.
Minimum inward air flow is 75 lfm.
Approximately 70% of air air is recirculated through the supply HEPA filter (D).
Must not be used with volatile toxic chemicals. Must not be hard-ducted to building exhaust system. Proper method of connecting Type A1 or A2 cabinets to building exhaust is through use of a canopy hood.
What does this illustrate?
What are the installation stipulations of this design?
Canopy (thimble) unit for ducting a Class II Type A BSC, which helps ensure that air flow of the building exhaust does not disturb internal cabinet flow.
Canopy must be removable or be designed to allow for operational testing of the cabinet. The airflow of the building exhaust must be sufficient to maintain the flow of room air into the gap between the canopy unit and the filter housing.
What is this?
Room air is drawn throught the face opening at what minimum inflow velocity?
What percentage of downflow air exits through the rear grille (E)?
Class II Type B1 BSC - designed for manipulations of minute quantities of hazardous chemicals with in vitro biological systems.
100 linear feet per minute (lfm)
70%
What is this?
lfm of inflow?
Amount of recirculated air?
Class II Type B1 BSC (benchtop design)
Same as upright design - 100 lfm
Same as upright design - 30% recirculated
What is this?
lfm of inflow?
Amount of recirculated air?
Class II Type B2
100 lfm
No recirculated air. 100% exhausted.
What is this?
inflow lfm?
Amount recirculated?
Can volatile toxic chemicals be used?
Radionuclides?
Class II Type A2 (formerly called Type B3)
100 lfm
Approximately 70%
Minute quantities of toxic chemicals or radionuclides can be used only if exhausts to outside via a canopy connection.
Meets requirements of Type B3 only if ducted to outdoors.
What is this?
Class III BSC - designed for work with highly infectious microbiological agents and for conduct of hazardous operations.
Gas-tight - no leak greater than 1 x 10-7 cc/sec with 1% test gas at 3 inches pressure water gauge.
Access for passage of materials into cabinet is through dunk tank or double-door box (e.g.autoclave)
Both supply and exhaust air are HEPA filtered. Exhaust air must pass through two HEPA filters and not exhausted through general laboratory exhaust system.
What is this?
Horizontal laminar flow “clean bench” - NOT a BSC
Only provides product protection. Discharges HEPA-filtered air from the back of the cabinet across the work surface and toward the user.
Can be used for certain clean activities, such as dust-free assembly of sterile equipment or electronic devices.
Should never be used when handling cell culture materials, drug formulations, potentially infectious materials, or any other potentially hazadous matrials.
What is this?
Vertical flow “clean bench” - not a BSC
May be useful for preparation of intravenous solutions. Product protection only.
If this cabinet is used to label infectious organisms with I125, what type of design must be used?
A modified Class I BSC with hard-ducting, Lexan® hinged doors (B), exhaust charcoal filter (C), and exhaust HEPA filter (D).
