Biological Molecules Flashcards
chemical elements in carbohydrates
carbon oxygen and hydrogen
chemical elements in protein
carbon oxygen hydrogen and nitrogen (some contain sulphur)
monosaccharide and examples
-a simple sugar (simplest)
-glucose or fructose
disaccharide and examples
-made when two monosaccharides join together
-maltose (2 glucose molecules)
polysaccharide and examples
-forms when lots of monosaccharides join together
-starch, glycogen and cellulose
-insoluble, useful as storage molecules
what are lipids made out of
most are:
-triglyceride, formed from one glycerol molecules chemically bonded to three fatty acids (acidic when broken up due to fatty acid)
-they are divided into two groups : fats (solid at room temp) and oils (liquid at room temp)
what are proteins formed from, and examples of proteins
-long chains of amino acids join together to form a protein
-differently arranged amino acids can form different proteins
-different proteins thus have different shapes, and the shape determines its function
-e.g. enzymes, haemoglobin, ligaments and keratin
steps to prepare a food sample
- break up the food using a pestle and mortar
- transfer to a test tube and add distilled water
- mix the food with water by stirring with a glass rod
- filter the mixture using a funnel and filter paper, collecting the solution (filtrate)
- proceed with the food tests
test for glucose
- add benedict’s solution to the sample solution in a test tube
- Heat in a boiling water bath for 5 minutes
3, take the test tube out and observe the colour - positive test changes solution from blue to green, yellow, orange and brick-red from low to high concentration
safety precautions for glucose test
-handle test tube with tongs
-wear safety googles, heatproof gloves
test for starch
- add food sample to a test tube
- add a few drops of iodine solution
- positive test shows colour change from orange-brown to blue-black
hazards in test for starch
-iodine is an irritant to the eyes, wear googles
-sodium hydroxide is in biuret solution, which is corrosive, wash skin if it touches your skin
protein food test
- add drops of biuret solution to the food sample
- positive test goes from blue to violet/purple
hazards in test for protein
biuret solution contains copper 2 sulfate which is dangerous if it gets in the eyes, wear googles
test for lipids (fats)
- mix the food sample with 4 (or 2)cm of ethanol and shake
- allow time for the sample to dissolve in the ethanol
- strain the ethanol solution into another test tube
- add the ethanol solution to an equal volume of cold distilled water 4 (or 2)cm
- a positive test will show a cloudy emulsion forming, colourless in negative test result
hazards in test for lipids
-ethanol is highly flammable, keep it away from bunsen burners
why is ethanol used in test for lipids
fats and oils are insoluble in water but will dissolve in ethanol
what are enzymes
-proteins that acts as biological catalysts to speed up the rate of a chemical reaction without being changed or used up in the reaction