bioenergetics Flashcards
photosynthesis, photosynthesis practical, respiration, aerobic & anaerobic respiration, effects of exercise, oxygen debt
photosynthesis word equation
carbon dioxide (air) + water (soil)
light
—————>
chlorophyll
glucose (storage/starch) + oxygen (waste gas; leaves thru stomata)
photosynthesis balanced symbol equation
6CO2 + 6H2O
light
—————–>
chlorophyll
C6H12O6 + 6O2
explanation of the photosynthesis equation
stage 1 (reactants) –>
reactants of water, carbon dioxide and sunlight enter the chloroplast.
the CO2 enters the leaves through the stomata; the water enters the plants through the roots and is transported to the leaves in the xylem.
here the chlorophyll absorbs light energy and converts it into energy that breaks apart the water molecules.
stage 2 (products) –>
the process moves into the connective tissue/cell and energy is used to break apart the carbon dioxide and assemble atoms into glucose - a sugar in plant food.
extra oxygen molecules are released as part of the plant’s waste gas.
testing leaves for starch
- half fill a boiling tube with hot water from the water bath and insert your leaf
- leave the leaf in there for 1 minute to rid the waxy cuticle coating
- pour away boiling water from tube
- cover the leaf with ethanol and put it back into the water bath
- leave for 5-10 minutes–> the ethanol will remove colour from the leaf because chlorophyll is soluble in the ethanol
- pour ethanol into a clean beaker and place leaf back into hot water to rinse off ethanol
- lay the leaf on a white tile and “flood” the leaf with iodine solution
- pour off excess iodine and examine the leaf to see where the starch is
HEALTHY LEAF - dark green and signs on starch
NO LIGHT LEAF - dark green and no sign of starch
photosynthesis required practical information (not method)
investigating the effect of light intensity on the rate of photosynthesis –> using carbon dioxide and water to produce glucose and oxygen
aquatic plants (PONDWEED) produce visible bubbles of oxygen gas into the surroundings when photosynthesising - bubbles can be counted as a measure of the rate of photosynthesis
effect of light intensity can be investigated by varying the distance between pondweed and a light source - closer light source, greater light intensity
as you move the lamp further away, the light intensity falls and so should the rate of photosynthesis
what is inverse square law
light intensity is inversely proportional to the distance between the light source and the experiment tube squared
relative light intensity
- 1/d squared
1 = mean bubbles produced per minute
d squared = distance between pondweed and light source
photosynthesis required practical method
INVENTORY:
- a boiling tube
- freshly cut 10 cm piece of pondweed
- a light source
- a ruler
- a test tube rack
- a stop watch
- 0.2% solution of sodium hydrogen carbonate
- a glass rod
METHOD:
1 - set up a test tube rack containing a boiling tube at a distance of 10cm away from the light source
2- fill the boiling tube with the sodium hydrogen carbonate solution
3 - put the piece of pondweed into the boiling tube with the cut end at the top. gently push the pondweed down with a glass rod
4 - leave the boiling tube for 5 minutes
5 - start the stopwatch and count the number of bubbles produced in one minute
6 - record results in a table:
distance|no. of bubbles/min|
_________|1____2____3____mean|
10______|_____|_____|___|_____|
20______|_____|_____|___|_____|
30______|_____|_____|___|_____|
40______|_____|_____|___|_____|
7 - repeat the count twice more and fill in the table to calculate the mean number of bubbles/minute
8 - repeat 1-7 with the test tube and boiling rackat distances of 20/30/40 cm away from the light source
what is respiration
- a chemical reaction and is catalysed by enzymes
- breaks bonds in glucose to release energy (energy stored)
- takes place in mitochondria
–> highly folded area for greater SA for respiration
respiration word equation
glucose + oxygen –> carbon dioxide + water
respiration symbol equation
C6H12O6 + 6O2 –> 6C02 + 6H2O
requirement of respiration in organisms
for the transfer from food eaten for cellular activities:
- movement -> muscle contraction
- building large molecules from smaller ones* -> synthesis
- thermoregulation - heat generation/reducing heat loss
- smaller molecules that you get from digestion being put back together to make larger molecules –> ATP ‘sticks’ them together
proteins –> amino acids
fats –> glycerol + fatty acids
starch –> glucose
<—————— you need energy
test for CO2
when carbon dioxide is bubbled through limewater (calcium hydroxide) it turns cloudy/milky
aerobic respiration information
occurs mostly mitochondria and a bit in cytoplasm
presence of oxygen: present - oxygen is used to break down food molecules in respiration
oxidation of glucose: complete - glucose is oxidised* to release its energy
–> glucose + oxygen → carbon dioxide + water + energy released
products of respiration: carbon dioxide and water - the products do not contain stored chemical energy
amount of energy released: relatively large amount
*chemical substances are oxidised by the addition of oxygen, removal of hydrogen or the removal of electrons.
anaerobic respiration information
occurs in cytoplasm
presence of oxygen: absent or in short supply - most organisms cannot respire without oxygen but some organisms and tissues can continue to respire if the oxygen runs out (humans can for a bit)
oxidation of glucose: incomplete, the products of respiration still contain energy -the glucose in muscle is converted to lactic acid
–> glucose → lactic acid + energy released
products of respiration: mammalian muscle: lactic acid
yeast: ethanol and carbon dioxide
some plants: ethanol and carbon dioxide
the products still contain stored chemical energy
amount of energy released: small amount but quickly
effects on exercise on the body
- heart rate increases
- the rate and depth of breathing increases
these changes increase blood flow to the muscles and so increase the supply of glucose, oxygen and removal of carbon dioxide
muscles store glucose as glycogen which can be converted back to glucose for the exercise
during exercise, aerobic and anaerobic use:
–> glucose is broken down into lactic acid
–> causes muscle fatigue/cramps/pain