Biochemistry Lab

Question 1

In the anaerobic glycolysis experiment, the optical density of the sample was measured as 100 at 420 nm wavelength, and the optical density of the standard was measured as 26.4. Accordingly, which of the following is the glucose concentration found in the sample? (Standard concentration (Cstd): 0.088, Dilution factor (F): 300)

a) 200
b) 400
c) 500
d) 100
e) 300

Answer 1

d) 100

Question 2

Which of the following is the method used for qualitative determination of glucose in urine in the glucose tolerance test?

a) Glucose oxidase
b) Millon
c) Edman
d) Benedict
e) Kjeldahl

Answer 2

d) Benedict

Question 3

In anaerobic glycolysis experiment: The colored compound obtained in the determination of phosphorus is measured spectrophotometrically in which of the following wavelengths.

a) 560
b) 410
c) 600
d) 340
e) 520

Answer 3

c) 600

Question 4

Which of the following is the reagent used for the formation of yellow colored product in the qualitative determination of lactic acid in the anaerobic glycolysis experiment?

a) Ba(OH)2
b) FeCl3
c) MgCl₂
d) ZnSO4
e) CuSO4

Answer 4

b) FeCl3

Question 5

Which of the following is the principle of Folin-Wu method used in determination of serum glucose in anaerobic glycolysis experiment?

a) Glucose forms a purple colored compound FeCl3
b) Glucose creates a pinkish color with copper sulphate in a basic environment
c) Glucose reduces Cu+2 to Cu+1 in an alkaline environment
d) Glucose forms a white colored precipitate with Ba(OH)2
e) Glucose forms yellow compound with ammonium molybdate

Answer 5

c) Glucose reduces Cu+2 to Cu+1 in an alkaline environment

Question 6

Which of the following is the diagnostic criteria for diabetes mellitus?

a) Fasting Plasma se ≥ 100mg / dL
b) In Oral Glucose Tolerance Test (OGTT), 2nd hour plasma glucose level ≥ 140 mg/dL
c) Randomly measured blood se ≥ 200mg / dL and accompanied by symptoms of diabetes mellutus
d) Presence of ketone bodies in urine
e) HbA1c level ≥ 5.4%

Answer 6

c) Randomly measured blood se ≥ 200mg / dL and accompanied by symptoms of diabetes mellutus

Question 7

kan şekeri diyagnoz ve takibinde kullanılan teknik?

Answer 7

HbA1c

Question 8

sonucu yanlış çıkan deneyde hangi aşamada yanlış yapmış olmalıyız?

Answer 8

the test tube was not pipetted with alkaline CuSO4

Question 9

kalıntılarını uzaklaştırmak için kulandığımız madde?

Answer 9

Ba(OH)2

Question 10

deneyin sonunda tüpte hangi ürünlerin oluşması beklenmez?

Atp

Amp and pi

Mg and k

Pyruvate

Answer 10

Pyruvate

Question 11

anaerobic deneyini inhibe etmek için hangisi kullanılır?

Mg

K

Ba(OH)2

CuSO4

Sulfiric acid

Answer 11

Ba(OH)2

Question 12

hangisi 2 saatin sonunda diabet teşhisi koymak için sınır kriterdir?

126

140

180

200

200>

Answer 12

200>

Question 13

hangisi doğru?

-lactic acid forms a yellow iron lactate with fecl3

Answer 13

..

Question 14

hangist yanlış?

• The blue color that occurs in the urine glucose assay indicates the high glucose concentration

Answer 14

..

Question 15

LAB sorusu,

• Coloring step was done with ANSA.

Answer 15

,,

Question 16

anaerobic glikolizle ilgili hangisi yanlıştır?

• The amount of glucose in 60-minute tube is greater than in the 0-minute tube.

Answer 16

..

Question 17

hangiler doğrudur?

II- Blood glucose determination is performed according to the Folin-wu method.

IV- Acetone determination in urine is done qualitatively.

Answer 17

..

Question 18

What is the principle of Folin-Wu method for determination of blood glucose?

A) Of Cu+1to Cu+2 reduction of glucose in alkaline environment

B) glucose increase with NaOH

C) in the acidic environment, glucose of Cu+click to Cu+³ oxidation

D) boiling up to the boiling point of glucose

E) precipitation with heavy metals

Answer 18

A) Of Cu+1to Cu+2 reduction of glucose in alkaline environment

Question 19

Which method is used for the determination of glucose concentration in the blood, reduced copper and phosphomolybdic acid colored complex formed?

A) Folin-Wu

B) Salivanof

C) Edman

D) Benedikct

E) Biüret

Answer 19

A) Folin-Wu

Question 20

Which is used for the determination of glucose in the urine test?

A) Folin-Wu

B) Biüret

C) Ninhidrin

D) Sanger

E) Benedict

Answer 20

E) Benedict

Question 21

The patient to make a diagnosis of pre-diabetes, OGTT glucose concentration for the second hour after glucose administration should be how?

A) 150-220 mg/dL

B) 100-120 mg/dL

C) 200-240 mg/dL

D) 140-199 mg/dL

E) 80-100 mg/dL

Answer 21

D) 140-199 mg/dL