Biochemistry Exam Chapter 2 Flashcards
What group is attached to the pyrimidine ring in thymine and is not present in uracil?
A) ribose
B) -CH3
C) -NH2
D) deoxyribose
B) -CH3
What is the purpose of DNA polymerase
it is used to replicate the DNA in the 5 to 3 direction.
Nucleoside triphosphates carry energy in the form of
phosphoanhydride bonds
In Sanger dideoxycytidine DNA sequencing, DNA polymerase 1 is used to add nucleotides to the_ end of the growing polynucleotide chain.
3’
Nucleotides play a central role in living organisms because:
A. They mediate transport of energy within the cell.
B. They are involved in oxidation-reduction reactions.
C. They are involved in intracellular signaling.
D. All of the above
All of the above
Phosphodiester bonds link DNA monomers to form a polymer bu which mechanism?
The OH group on C-3 in the 2 deoxyribose carbohydrate forms a covalent bond to elongate the DNA chain.
Inside our cells, free nucleotides are almost always associated with ______.
A) proteins
B) cholesterol
C) Cl− counterions
D) Mg2+ counterions
D) Mg2+ counterions
Dideoxy analogs are used in Sanger sequencing for what reason?
They block further elongation of the DNA
What the of bond is made between nucleotides
phosphodiester
In most organisms, replication proceeds in a _ manner from the_
bidirectional, replication origin
Knowledge about the tautomeric forms of the bases of nucleic acids is needed
to understand the H Bonding between the complementary bases
Which of the following correctly describes the B-DNA double helix?
All of the above ( antiparallel, right hand, base pairs, and one helical)
Polypeptide synthesis proceeds from the _ to the _
N Terminus, C terminus
Which of the following statements is true regarding phophodiester bonds that link to RNA and DNA?
Phosphodiester bonds join the 3 hydroxyl of one nucleotide to the 5 Hydrogen of the next
