Biochemistry Chapter 5: Analytic Techniques

Question 1

In gel electrophoresis, molecules move from

Answer 1

Cathode (-) to anode (+)

Question 2

True or false: PCR can be used to quantify gene expression

Answer 2

False. Quantifying gene expression must be performed with other lab techniques, such as qPCR/RT-PCR.

Question 3

what does PCR require?

Answer 3

Forward and reverse primers
Taq polymerase
A magnesium containing buffer

Question 4

True or false: retention factor can only be between 0 - 1

Answer 4

True. Its Rf value will thus be quite high. Rf, or the retention factor, is equal to the distance a given solute has migrated up the stationary phase, divided by the maximum distance traveled by the mobile phase, or solvent. This means that Rf can range from 0 to 1; lower Rf values (closer to 0) are associated with more polar compounds, whereas higher values (closer to 1) are associated with more nonpolar compounds.

Question 5

Rf formula

Answer 5

Question 6

Beer lambert law

Answer 6

where A is absorptivity, ε is the molar attenuation coefficient, and c is the concentration. Essentially, it indicates that higher levels of absorbance mean that more light is absorbed, which means that more of a substance is present.

Question 7

technique that assesses the concentration of protein by measuring the extent to which the unlabeled proteins in the sample compete with radioactively labeled antigens for antibody binding sites

Answer 7

Radioimmunoassay

Question 8

technique in which antibodies bind antigens, and then the amount of bound antibody is determined by reactions that produce a visible signal, like color or fluorescence

Answer 8

ELISA

Question 9

Rule of thumb for correlation between variables

Answer 9

Question 10

Fractional distillation is used to separate what kind of compounds?

Answer 10

Compounds with very close boiling points