Biochemical Tests

Question 1

Urease
- what is it for?
- pH indicator?
- positive result
- negative result

Answer 1

1) identifies organisms that can hydrolyze urea to produce ammonia and carbon dioxide
2) phenol red
3) negative - yellow in acidic environment
4) positive - fuschia in alkaline enviornment

Question 2

Citrate
- what is it used for?
- negative result
- positive result

Answer 2

1) sees if bacteria uses citrate as carbon source during oxidative metabolism
2) negative - green
3) positive - blue

Question 3

Duram Lactose
- what is it used for?
- negative result
- positive result

Answer 3

1) lactose is the sole carbohydrate will find out if any fermentation occurs
2) negative - reddish pink
3) positive - yellow with air bubble