Lab Final Flashcards
Name the 2 ways to measure bacterial concentration
1) Spectrophotometer with turbidity
2) SPC Plates
If a solution is more concentrated will that be greater or less turbidity?
More turbid
If a solution is more turbid will fewer or greater amounts of light scatter?
More light scatters
%T is higher when cell count is higher or lower?
Lower
Absorbance is higher when cell count is higher or lower?
Higher
Kirby Bauer Test is designed for what?
Test antibiotic susceptibility
Disk filter method of zone of inhibition is used for what?
Tests for antiseptic susceptibility
Is blood agar differential or selective?
Only differential towards those that can lyse red blood cells
Alpha hemolysis looks and means what?
Murky green - partial lysis
Beta hemolysis looks and means what?
Clear - complete lysis
Gamma lysis looks and means what?
No change - no lysis
Asperigillus Fungi
Deuteromycota asexual
Penicillium Fungi
Deuteromycota asexual
Penicillium Fungi
Deuteromycota asexual
Rhizopus Zygospore
Zygomycota sexual
Sporangiospore
Zygomycota asexual
Yeasts are (uni or multicellular, have filaments?, shape, reproduction)?
- unicellular
- no filaments
- spherical or oval shape
- reproduce asexually
Molds are (uni/multicellular, have filaments?, have hyphae, examples)
- multicellular
-filaments - hyphae
- shrooms and large bracket
Gram Staining 4 steps
1) crystal violet
2) iodine
3) decolorizer
4) safranin
Which 2 bacteria can produce endospores?
Clostridium and bacillus
Endospore procedure
1) malachite green
2) steam slide 10 min
3) rinse with water
4) safranin
Endospore procedure
1) malachite green
2) steam slide 10 min
3) rinse with water
4) safranin
