Lab Final Flashcards

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1
Q

Name the 2 ways to measure bacterial concentration

A

1) Spectrophotometer with turbidity
2) SPC Plates

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2
Q

If a solution is more concentrated will that be greater or less turbidity?

A

More turbid

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3
Q

If a solution is more turbid will fewer or greater amounts of light scatter?

A

More light scatters

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4
Q

%T is higher when cell count is higher or lower?

A

Lower

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5
Q

Absorbance is higher when cell count is higher or lower?

A

Higher

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6
Q

Kirby Bauer Test is designed for what?

A

Test antibiotic susceptibility

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7
Q

Disk filter method of zone of inhibition is used for what?

A

Tests for antiseptic susceptibility

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8
Q

Is blood agar differential or selective?

A

Only differential towards those that can lyse red blood cells

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9
Q

Alpha hemolysis looks and means what?

A

Murky green - partial lysis

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10
Q

Beta hemolysis looks and means what?

A

Clear - complete lysis

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11
Q

Gamma lysis looks and means what?

A

No change - no lysis

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12
Q

Asperigillus Fungi

A

Deuteromycota asexual

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13
Q

Penicillium Fungi

A

Deuteromycota asexual

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14
Q

Penicillium Fungi

A

Deuteromycota asexual

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15
Q

Rhizopus Zygospore

A

Zygomycota sexual

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16
Q

Sporangiospore

A

Zygomycota asexual

17
Q

Yeasts are (uni or multicellular, have filaments?, shape, reproduction)?

A
  • unicellular
  • no filaments
  • spherical or oval shape
  • reproduce asexually
18
Q

Molds are (uni/multicellular, have filaments?, have hyphae, examples)

A
  • multicellular
    -filaments
  • hyphae
  • shrooms and large bracket
19
Q

Gram Staining 4 steps

A

1) crystal violet
2) iodine
3) decolorizer
4) safranin

20
Q

Which 2 bacteria can produce endospores?

A

Clostridium and bacillus

21
Q

Endospore procedure

A

1) malachite green
2) steam slide 10 min
3) rinse with water
4) safranin

22
Q

Endospore procedure

A

1) malachite green
2) steam slide 10 min
3) rinse with water
4) safranin