Biochemical Pathways and Complementation Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What is a biochemical pathway?

A

A series of steps involving several intermediate product being transformed into other things by enzymes until the end product necessary for life is created

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

How can a prototrophic strain be distinguished from an auxotrophic strain?

A

Prototrophs will grow on minimal media, but auxotrophs won’t

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Why are auxotrophs unable to grow on minimal media?

A

They have a blockage in a pathway that prevents them from making some essential end product. Without the end product, they can’t grow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

How can an auxotrophic strain be rescued?

A

By adding an intermediate product downstream of the blockage or by adding the end product

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What did Beadle and Tatum’s experiment demonstrate about biochemical pathways?

A

A mutation in a single gene disrupts the function of a single enzyme, so one gene, one enzyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is complementation?

A

The restoration of wild type phenotype when two haploid mutants are crossed

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the difference between allelic and non-allelic mutations?

A

Allelic mutations are on the same gene, non-allelic mutations are on different genes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What does a complementation test tell us?

A

If two auxotrophic strains with the same mutant phenotype have allelic or non-allelic mutations

How well did you know this?
1
Not at all
2
3
4
5
Perfectly