Biochem of nucleic acids

Question 1

3D folded shape of nucleic acid

Answer 1

Tertiary structure

Question 2

Also called molecular scissors

Answer 2

Restriction enzymes

Question 3

Also called annealing helicase

Answer 3

Ligase

Question 4

Also called continuous strand

Answer 4

Leading strand

Question 5

Creates the sugar phosphate backbone

Answer 5

Phosphodiester bonds

Question 6

Diameter of B-DNA

Answer 6

2.37nm

Question 7

Difference of nucleoside and nucleotide

Answer 7

Nucleotide includes the phosphate while nucleoside only includes sugar and base

Question 8

Does purine complement pyrimidine?

Answer 8

Yes

Question 9

Enzyme that builds a new strand

Answer 9

DNAP

Question 10

Formed by the hydrogen bonds that links a purine and a pyrimidine

Answer 10

Double helix

Question 11

Fulfill the gaps between fragments

Answer 11

Ligase

Question 12

Guanine is a pyrimidine

Answer 12

False

Question 13

It is the opposing orientation of the two nucleotide chains in a DNA molecule

Answer 13

Antiparalellism

Question 14

Ligase can be used in recombination

Answer 14

True

Question 15

Most commonly used restriction enzyme in the laboratory

Answer 15

Type 2

Question 16

Pyrimidine has a single ring structure

Answer 16

True

Question 17

Step 3 of DNA replication

Answer 17

Primase makes the DNA template

Question 18

Two types of helicase

Answer 18

Topoisomerase

Gyrase (Type 2 topoisomerase)

Question 19

Structure of DNA wherein we can see the supercoiling of DNA

Answer 19

Quaternary structure

Question 20

Amino acids are linked together by

Answer 20

Peptide bonds

Question 21

Biggest type of RNA

Answer 21

mRNA

Question 22

Codes for protein called repressor

Answer 22

LacI

Question 23

Extra chromosomes that carry genetic information

Answer 23

Plasmids

Question 24

Gyrase only cuts one side of the strand

Answer 24

False

Question 25

Importance of pre mRNA processing

Answer 25

Protection of mRNA from degradation while being exported out of the nucleus

Question 26

Inhibited by high concentration of a-amanitin

Answer 26

Type 3 RNA polymerase

Question 27

Operates the transcription process

Answer 27

Operons

Question 28

Plasmids are included in the DNA replication of bacterial cells

Answer 28

False

Question 29

Recombination in eukaryotic cells occur in what process

Answer 29

Meiosis

Question 30

Removal of intron sequences in mRNA that do not encode functional proteins

Answer 30

Splicing

Question 31

rRNA is the smallest RNA

Answer 31

False

Question 32

Stop codon called opal

Answer 32

UGA

Question 33

Transfer amino acids to mRNA for protein synthesis

Answer 33

tRNA

Question 34

Types of RNA

Answer 34

``` mRNA tRNA rRNA snRNA siRNA miRNA ```

Question 35

What type of RNA polymerase produces tRNA

Answer 35

Type 3

Question 36

Where can type 1 RNa polymerase be found

Answer 36

Nucleolus

Question 37

Which group dictates the nature of amino acid

Answer 37

R group