Molecular biology and diagnostics Flashcards
1
Q
initial routine laboratory procedures for DNA isolation
A
Density gradient Centrifugation strategies
2
Q
3 steps of isolation strategy
A
Separation of target cells/viruses
Release of cellular material
Target nucleic acid is purified
3
Q
2 types of isolating WBCs
A
Isolation by differential density gradient centrifugation
By differential lysis
4
Q
T or F:
Xylene is a mixture of four isomers of dimethylbenzez
A
False; 3 isomers
5
Q
T or F:
Organic isolation methods is also known as salting out
A
False; Inorganic
6
Q
how many percent of total RNA is rRNA
A
80-90%
7
Q
fluorescent dyes used in electrophoresis
A
Ethidium bromide
Sybre green
