biochem lab

Question 1

Xanthoproteic test

Answer 1

orange color, conc. HNO3, NaOH, Tyrosine
orange color, conc. HNO3, NaOH, Tryptophan

Question 2

Millons test

Answer 2

Red color, Tyrosine

Question 3

Hopkin’s cole test

Answer 3

Violet Ring, conc.H2so4, Glycooxylic acid, Tryptophan

Question 4

Ninhydrin Test

Answer 4

Blue color ONLY

Question 5

Sakaguchi Test

Answer 5

Red color, alkaline solution, alpha nalphtol, sodium hypochloride

Question 6

A solution that has the same salt concentration (0.9%) as cells and blood

Answer 6

Isotonic solution

Question 7

A solution that has lower osmotic pressure than another solution to which it is compared

Answer 7

Hypotonic solution

Question 8

movement of fluid of higher water concentration to lower water concentration

Answer 8

Osmosis

Question 9

Mucic test is used to indicate the presence of

Answer 9

Lactose & Galactose

Question 10

General color test for carbohydrates with their positive results

Answer 10

Molisch test (Violet ring is formed at the junction of 2 layers)
Anthrone test (blue green color complex)

Question 11

water

Answer 11

soluble

Question 12

0.1m NaOH

Answer 12

Slight soluble

Question 13

0.1m HCl

Answer 13

soluble

Question 14

Ethyl alcohol

Answer 14

Slight soluble

Question 15

chlorofoam

Answer 15

insoluble

Question 16

to form regeants

Answer 16

2-3 chemicals

Question 17

Tests for reducing sugars

Answer 17

Benedicts test (brick red ppt)
Nylanders Test (black ppt)
Barfoeds test (brick red ppt)
Fehlings Test (brick red ppt)

Question 18

what is formed in positive Mucic test

Answer 18

crystal

Question 19

what is formed in positive Mucic test

Answer 19

crystal

Question 20

Seliwanoffs test is for?

Answer 20

Ketones (cherry red colored complex formation)

Question 21

chemical composition of Seliwanoff’s reagent

Answer 21

Resorcino conc.
HCl

Question 22

Result for Aniline Acetate Test

Answer 22

Bright pink color is produced in filter paper

Question 23

Tests for Pentoses

Answer 23

Aniline acetate test
Bials test

Question 24

Result for bials test

Answer 24

Blue green color complex from

Question 25

chemical composition of bials reagent

Answer 25

orcinol HCl Ferric Chloride (FeCl3)

Question 26

hydrates of carbon

Answer 26

Carbohydrates

Question 27

carbohydrates are compound of ______ , _____

Answer 27

polyhydroxyaldehyde polyhydroxyketone

Question 28

are product of photosynthesis

Answer 28

carbohydrates

Question 29

general formula of carbohydrates

Answer 29

CnH2n / Cn(H2)n

Question 30

building block of nucleic acids

Answer 30

nucleotides

Question 31

building block of carbohydrates

Answer 31

Saccharide

Question 32

contains sugar and base

Answer 32

nucleosides

Question 33

contains sugar base and phosphate

Answer 33

Nucleotides

Question 34

most important monosaccharide found in plants

Answer 34

Hexose (glu/gal/fruc)

Question 35

aldo-hexose blood sugar, grape sugar, physiological sugar

Answer 35

Glucose

Question 36

keto-hexose fruit sugar / sugar cane

Answer 36

Fructose

Question 37

aldo pentose

Answer 37

ribose

Question 38

3 classes of carbohydrates

Answer 38

monosaccharides oligosaccharides polysaccharides

Question 39

1 sugar unit

Answer 39

monosaccharide

Question 40

2-10 sugar units

Answer 40

Oligosaccharides

Question 41

more than 10 sugar units polymer of monosacharrides

Answer 41

Polysaccharide

Question 42

chemical test to detect presence of monosacharides. based on the reduction of Cupric II Acetate to CUPROUS I OXIDE

Answer 42

Barfoed Test (Brick red ppt)

Question 43

the presence of alkaline sodium carbonates convert sugar into strong reducing agent called "ENEDIOLS"

Answer 43

Benedicts test

Question 44

during reduction reaction, the color will change from blue to brick red ppt. due to presence of CUPROUS I OXIDE

Answer 44

Benedicts test

Question 45

cell to swell

Answer 45

hypotonic

Question 46

cell shrink

Answer 46

hypertonic

Question 47

destruction of rbc

Answer 47

hemolysis

Question 48

rbc shrinking

Answer 48

Crenation

Question 49

essential amino acids

Answer 49

Lysine Leucine Isoleucine Methionine Phenylalanine Histidine Threonine Tryptophan Valine