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MT - BioLec > biochem lab > Flashcards

biochem lab Flashcards

1
Q

Xanthoproteic test

A

orange color, conc. HNO3, NaOH, Tyrosine
orange color, conc. HNO3, NaOH, Tryptophan

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2
Q

Millons test

A

Red color, Tyrosine

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3
Q

Hopkin’s cole test

A

Violet Ring, conc.H2so4, Glycooxylic acid, Tryptophan

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4
Q

Ninhydrin Test

A

Blue color ONLY

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5
Q

Sakaguchi Test

A

Red color, alkaline solution, alpha nalphtol, sodium hypochloride

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6
Q

A solution that has the same salt concentration (0.9%) as cells and blood

A

Isotonic solution

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7
Q

A solution that has lower osmotic pressure than another solution to which it is compared

A

Hypotonic solution

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8
Q

movement of fluid of higher water concentration to lower water concentration

A

Osmosis

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9
Q

Mucic test is used to indicate the presence of

A

Lactose & Galactose

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10
Q

General color test for carbohydrates with their positive results

A

Molisch test (Violet ring is formed at the junction of 2 layers)
Anthrone test (blue green color complex)

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11
Q

water

A

soluble

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12
Q

0.1m NaOH

A

Slight soluble

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13
Q

0.1m HCl

A

soluble

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14
Q

Ethyl alcohol

A

Slight soluble

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15
Q

chlorofoam

A

insoluble

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16
Q

to form regeants

A

2-3 chemicals

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17
Q

Tests for reducing sugars

A

Benedicts test (brick red ppt)
Nylanders Test (black ppt)
Barfoeds test (brick red ppt)
Fehlings Test (brick red ppt)

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18
Q

what is formed in positive Mucic test

A

crystal

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19
Q

what is formed in positive Mucic test

A

crystal

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20
Q

Seliwanoffs test is for?

A

Ketones (cherry red colored complex formation)

21
Q

chemical composition of Seliwanoff’s reagent

A

Resorcino conc.
HCl

22
Q

Result for Aniline Acetate Test

A

Bright pink color is produced in filter paper

23
Q

Tests for Pentoses

A

Aniline acetate test
Bials test

24
Q

Result for bials test

A

Blue green color complex from

25
Q

chemical composition of bials reagent

A

orcinol
HCl
Ferric Chloride (FeCl3)

26
Q

hydrates of carbon

A

Carbohydrates

27
Q

carbohydrates are compound of ______ , _____

A

polyhydroxyaldehyde
polyhydroxyketone

28
Q

are product of photosynthesis

A

carbohydrates

29
Q

general formula of carbohydrates

A

CnH2n / Cn(H2)n

30
Q

building block of nucleic acids

A

nucleotides

31
Q

building block of carbohydrates

A

Saccharide

32
Q

contains sugar and base

A

nucleosides

33
Q

contains sugar base and phosphate

A

Nucleotides

34
Q

most important monosaccharide found in plants

A

Hexose (glu/gal/fruc)

35
Q

aldo-hexose
blood sugar, grape sugar,
physiological sugar

A

Glucose

36
Q

keto-hexose
fruit sugar / sugar cane

A

Fructose

37
Q

aldo pentose

A

ribose

38
Q

3 classes of carbohydrates

A

monosaccharides
oligosaccharides
polysaccharides

39
Q

1 sugar unit

A

monosaccharide

40
Q

2-10 sugar units

A

Oligosaccharides

41
Q

more than 10 sugar units
polymer of monosacharrides

A

Polysaccharide

42
Q

chemical test to detect presence of monosacharides. based on the reduction of Cupric II Acetate to CUPROUS I OXIDE

A

Barfoed Test (Brick red ppt)

43
Q

the presence of alkaline sodium carbonates convert sugar into strong reducing agent called “ENEDIOLS”

A

Benedicts test

44
Q

during reduction reaction, the color will change from blue to brick red ppt. due to presence of CUPROUS I OXIDE

A

Benedicts test

45
Q

cell to swell

A

hypotonic

46
Q

cell shrink

A

hypertonic

47
Q

destruction of rbc

A

hemolysis

48
Q

rbc shrinking

A

Crenation

49
Q

essential amino acids

A

Lysine
Leucine
Isoleucine
Methionine
Phenylalanine
Histidine
Threonine
Tryptophan
Valine

MT - BioLec (3 decks)

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