bio lab 4

Question 1

parts of gene

Answer 1

regulatory sequence, exon, intron, exon, intron, exon, regulatory sequence

Question 2

exon

Answer 2

DNA that is translated to form a protein

Question 3

intron

Answer 3

DNA that does not get translated to form a protein

Question 4

transposon

Answer 4

gene that copies itself and move to different spots in the genome

Question 5

transposon move to exon

Answer 5

PHENOTYPE CHANGE

Question 6

transposon move to intron

Answer 6

NO PHENOTYPE CHANGE

Question 7

3 steps of polymerase chain reaction (PCR)

Answer 7

denaturing, annealing, extension

Question 8

denaturing

Answer 8

stranded DNA is separated into 2 strands

Question 9

annealing

Answer 9

primers bind to the beginning and end of DNA

Question 10

extension

Answer 10

DNA polymerase extends the 3 end of primer along the template stand

Question 11

purpose of detergents

Answer 11

helps in cell disruption and solubilize hydrophobic compounds

Question 12

purpose of protease

Answer 12

enzyme that helps cell membrane disruption by digesting proteins

Question 13

purpose of ethanol

Answer 13

ethanol causes DNA to precipitate into long clumps and leave other cellular components

Question 14

purpose of AW

Answer 14

contains ethanol so DNA remains while washing away cellular components

Question 15

purpose of AE

Answer 15

AE has no ethanol so DNA will be back into solution

Question 16

centrifuges

Answer 16

allows DNA to be back into solution through AE and pass through the spin filter into collection tube

Question 17

spin column

Answer 17

retains the precipitated DNA in the filter and compounds are forced into collection tube