Bio/Biochem Flashcards
Prokaryotes vs. Eukaryotes
Prokaryotes do NOT have membrane-bound organelles (such as ER, nucleus, etc). However, both Prokaryotes and Eukaryotes have a cell membrane and for some, a cell wall.
Describe the steps of reverse transcription
In reverse transcription: 1) single stranded RNA is used as a template to generate a complementary DNA strand. This step requires a RNA-dependent DNA Polymerase. 2) There is temporarily an RNA-DNA double helix, but the RNA strand is degraded, and the DNA strand is used as a template to generate a complementary DNA strand to form a DNA double helix. This step requires a DNA-dependent DNA Polymerase.
Megakaryocytes
Large bone marrow cells with a large nucleus that are responsible for the production of platelets necessary for normal blood clotting.
What is a key difference between viral genetics and eukaryotic genetics?
Viruses are NOT diploid, meaning they have ONE copy of a gene/allele.
What is the “fluid mosaic” model referring to
The fluid mosaic model is referring to two specific properties of the plasma membrane: 1) “Fluid” is the ability of certain molecules to travel laterally within the bilayer. 2) “Mosaic” refers to the presence of proteins and lipids/ other molecules scattered in its structure.
How do double bonds impact fluidity of plasma membrane?
More double bonds, more fluid a membrane is due to kinks.
Lytic vs. Lysogenic cycle
In the lytic cycle, the bacteriophage actively copies its genetic material, resulting in the lysing of the host cell. While, lysogenic cycle is a dormant stage, where the bacteriophage incorporates its DNA in the host genome to be activated later.
What is the impact of a stimulant, like cocaine, on glucose metabolism?
Since a stimulant such as cocaine, stimulates the CNS, it increases glucose metabolism in the brain. This effect is similar to the effect of stress!
Sympatric speciation
When reproductive isolation occurs without physical barriers
Allopatric speciation
When physical barriers cause reproductive isolation
Genetic bottleneck
When a reduction in the gene pool diversity occurs due a REDUCTION in population SIZE
Non-sense vs. missense mutation
Non-sense mutation occurs due to a premature STOP codon (UAG, UGA, UAA). Missense mutation is when one amino acid replaces another.
Adaptive radiation
When distinct species that play distinct ecological roles arise from a single ancestor
Five conditions of Hardy-Weinberg Equilibrium
- Large breeding population
- Random mating
- No immigration
- No mutations that change overall allele frequencies
- No natural selection
What direction does water move towards in osmosis?
Water moves towards greater osmotic pressure
Function of the Loop of Henle
Loop of Henle is a structure of nephrons (filtering cells in the kidney) that rely on Sodium pump to reabsorb sodium ions and make concentrated urine.
What Histone proteins are present in human nucleosomes?
H1, H2A, H3, and H4 are histones present in human nucleosomes. However, H1 is outside the bead structure of histones, because their role is to hold the DNA in place & provide stability for the structure.
What is the difference between Chromatin and nucleosomes?
Nucleosomes refers to the DNA-Histone subunits, while Chromatin is fully-packaged DNA.
How are euchromatin and heterochromatin viewed under a light microscope?
The lightly-packaged euchromatin is viewed lighter under a light microscope, while the more condensed heterochromatin is viewed darker.
What is the shape of a rate vs. pH graph for enzymes?
Parabolic- indicated that enzyme activity is optimal at a certain pH, but once the optimal pH is surpassed, the enzyme declines in function.
How is urea formed?
Urea is formed through amino acid metabolism. An enzyme called aminotransferase, replaces the amine group with a carboxyl or carbonyl group, and the free amine group becomes ammonia (NH3). Ammonia is a toxic metabolite that the liver and kidneys transfer to urea (CH4N2O) through combining it with CO2.
What direction do charged AAs travel in an isoelectric gel?
Positively charged AAs move towards cathode, while negatively charged AAs move towards anode.
Homologous Structures
Evolved from a common ancestor but now serve a different function (Ex: forelimbs of mammals such as humans, walruses, bats)
Analogous structure
Evolved individually but serve similar functions (Ex: wings of insects and bats)